Meiring Tracy L, Mbulawa Zizipho Z A, Lesosky Maia, Coetzee David, Williamson Anna-Lise
Division of Medical Virology, Department of Pathology and Institute of Infectious Diseases & Molecular Medicine, Faculty of Health Sciences, University of Cape Town, Cape Town 7925, South Africa.
Division of Medical Virology, Department of Pathology and Institute of Infectious Diseases & Molecular Medicine, Faculty of Health Sciences, University of Cape Town, Cape Town 7925, South Africa; Center for HIV & STIs, National Institute for Communicable Diseases, National Health Laboratory Service, Cape Town 7925, South Africa.
Papillomavirus Res. 2017 Jun;3:160-167. doi: 10.1016/j.pvr.2017.05.001. Epub 2017 May 10.
This study investigated the prevalence of HPVs in heterosexual South African men and the impact of HIV co-infection.
HPV was detected in penile swabs from 195 HIV-infected and 140 HIV-uninfected men using PCR with FAP59/64 primers and Roche Linear Array HPV genotyping (LA). Genotyping of FAP positive specimens was achieved by high-throughput sequencing of amplicons.
HPV was detected by FAP PCR and LA in 79% (266/335) of the men. Men with HIV co-infection and men with HIV infected sexual partners had a significantly (p<0.0001) higher HPV infection risk (adjusted odds ratio 4.0 (2.1-8.2) and 3.7 (2.1-6.7), respectively). LA genotyping and 454 sequencing of 218 FAP positive specimens detected 45 known α-HPV types, 45 β-HPV types (34 known, 10 putative and 1 novel putative), and 91 γ-HPV types (26 known, 51 putative and 14 novel putative). Alpha, beta and gamma types were detected in 89.8%, 51.4% and 62.4% of the 218 men with HPV-62, HPV-5 and HPV-121 most common in each genus, respectively.
A great diversity of known and novel alpha, beta and gamma HPV types were detected with higher prevalence in HIV co-infected men and unknown associations, if any, with genital lesions and cancers.
本研究调查了南非异性恋男性中HPV的流行情况以及HIV合并感染的影响。
使用FAP59/64引物的PCR和罗氏线性阵列HPV基因分型（LA），在195名HIV感染男性和140名未感染HIV的男性的阴茎拭子中检测HPV。通过扩增子的高通量测序对FAP阳性标本进行基因分型。
通过FAP PCR和LA在79%（266/335）的男性中检测到HPV。合并感染HIV的男性和有感染HIV性伴侣的男性HPV感染风险显著更高（p<0.0001）（调整后的优势比分别为4.0（2.1 - 8.2）和3.7（2.1 - 6.7））。对218个FAP阳性标本进行LA基因分型和454测序，检测到45种已知的α - HPV型、45种β - HPV型（34种已知、10种推定和1种新的推定）以及91种γ - HPV型（26种已知、51种推定和14种新的推定）。在218名感染HPV的男性中，分别有89.8%、51.4%和62.4%检测到α、β和γ型，各属中最常见的分别是HPV - 62、HPV - 5和HPV - 121。
检测到大量已知和新的α、β和γ型HPV，在合并感染HIV的男性中患病率更高，与生殖器病变和癌症的关联（若有）未知。
