Yu Bo-Han, Zhou Qian, Wang Zuo-Lin
Department of Stomatology, Tongji University, PR China.
Center of Implant Dentistry, Hospital of Stomatology, Tongji University, Shanghai, China
J Biomater Appl. 2014 Aug;29(2):243-253. doi: 10.1177/0885328214521846. Epub 2014 Jan 30.
The aim of this study was to compare the osteogenic effects of periodontal ligament stem cells (PDLSCs) versus bone marrow mesenchymal stem cells (BMMSCs) in combination with Bio-Oss scaffolds on subcutaneous and critical-size defects in the immunodeficient rat calvarium. PDLSCs and BMMSCs were obtained from the same canine donor. Twenty-four rats were randomly assigned to one of four experimental groups (n = 6 each): group A (no-graft negative control), group B (Bio-Oss positive control), group C (BMMSC/Bio-Oss test group), and group D (PDLSC/Bio-Oss test group). Eight weeks post-transplantation, ectopic and in situ bone regeneration was evaluated by micro-computed tomography (µ-CT), histology, histomorphometry, and immunohistochemistry. The stem cell/Bio-Oss constructs were significantly superior to the controls in terms of their ability to promote osteogenesis (p < 0.01), while the PDLSC/Bio-Oss construct tended to be superior to the BMMSC/Bio-Oss construct. Thus, engineered stem cell/Bio-Oss complexes can successfully reconstruct critical-size defects in rats, and PDLSCs and BMMSCs are both suitable as seed cells.
本研究旨在比较牙周膜干细胞（PDLSCs）与骨髓间充质干细胞（BMMSCs）联合Bio-Oss支架对免疫缺陷大鼠颅骨皮下和临界尺寸缺损的成骨作用。PDLSCs和BMMSCs取自同一只犬类供体。24只大鼠被随机分为四个实验组之一（每组n = 6）：A组（无移植阴性对照组）、B组（Bio-Oss阳性对照组）、C组（BMMSC/Bio-Oss试验组）和D组（PDLSC/Bio-Oss试验组）。移植后8周，通过微计算机断层扫描（µ-CT）、组织学、组织形态计量学和免疫组织化学评估异位和原位骨再生情况。干细胞/Bio-Oss构建体在促进成骨能力方面明显优于对照组（p < 0.01），而PDLSC/Bio-Oss构建体往往优于BMMSC/Bio-Oss构建体。因此，工程化干细胞/Bio-Oss复合物能够成功修复大鼠的临界尺寸缺损，PDLSCs和BMMSCs均适合作为种子细胞。
