Almdahl S M, Seljelid R
Dept. of Surgery, University Hospital, Tromsø, Norway.
Res Exp Med (Berl). 1987;187(5):369-77. doi: 10.1007/BF01855663.
The first part of this study in the rat was designed to assess the immediate and, in particular, the long-term effects of semisoluble aminated glucan (SAG) with regard to an intraperitoneal (i.p.) E. coli challenge and side-effects. The severity of the E. coli peritonitis was evaluated by quantification of the concomitant bacteremia. The animals randomly received either 10 ml normal saline i.p. (controls) or 10 ml (50 mg) SAG i.p. (experimental groups). It was found that SAG had no immediate protective effect against the infectious challenge as no difference was detected in the bacterial clearance between saline- and SAG-treated rats at day 0. However, 2 days after injection of saline/SAG a substantial protection of SAG was noted as no blood-borne bacteria could be detected at 1, 2, 3, 4 and 5 h after the E. coli challenge in SAG-treated animals, whereas by contrast matching blood cultures were positive in all controls. A likewise protective effect against E. coli bacteremia of a single i.p. injection of SAG was demonstrated at 7, 21, and 49 days after the administration of the compound. During the 7-week experimental period no clinical side-effects of SAG were observed. At autopsy (days 0, 2, 7, 21, and 49, respectively) no gross pathologic conditions were found. Lung, spleen, and kidney sections from SAG-treated rats were normal, whereas tiny granulomas were exhibited in liver sections, possibly related to the hyperfunctional macrophage state which SAG induced. The second main object of the study was to evaluate SAG's effect on adhesion formation.(ABSTRACT TRUNCATED AT 250 WORDS)
本研究在大鼠身上进行的第一部分旨在评估半溶性胺化葡聚糖（SAG）针对腹腔内注射大肠杆菌所产生的即时影响，尤其是长期影响，以及副作用。通过对伴随菌血症的量化来评估大肠杆菌性腹膜炎的严重程度。动物被随机分为两组，一组腹腔注射10毫升生理盐水（对照组），另一组腹腔注射10毫升（50毫克）SAG（实验组）。结果发现，SAG对感染性攻击没有即时保护作用，因为在第0天，生理盐水处理组和SAG处理组大鼠的细菌清除率没有差异。然而，在注射生理盐水/SAG两天后，发现SAG有显著的保护作用，因为在SAG处理的动物中，大肠杆菌攻击后1、2、3、4和5小时均未检测到血源细菌，而相比之下，所有对照组的配对血培养均为阳性。在给予该化合物7天、21天和49天后，单次腹腔注射SAG对大肠杆菌菌血症同样具有保护作用。在为期7周的实验期内，未观察到SAG的临床副作用。在尸检时（分别在第0天、第2天、第7天、第21天和第49天）未发现明显的病理状况。SAG处理组大鼠的肺、脾和肾切片均正常，而肝切片中出现了微小的肉芽肿，可能与SAG诱导的巨噬细胞功能亢进状态有关。该研究的第二个主要目的是评估SAG对粘连形成的影响。（摘要截选至250字）
