Almdahl S M, Bøgwald J, Hoffman J, Giercksky K E, Seljelid R
Eur Surg Res. 1987;19(2):78-85. doi: 10.1159/000128684.
The effect of prophylactic intraperitoneal aminated glucan on the survival rate and formation of adhesions and abscesses was investigated in rats with acute and subacute peritonitis, respectively induced by cecal perforation and ileal ligation. A significantly reduced mortality was found in both forms of peritonitis. Pretreatment by aminated glucan also significantly reduced the number of abscesses and peritoneal adhesions. An about threefold increase in peritoneal macrophages in aminated glucan-treated rats compared to controls was noted. In vitro studies, using 32P-labelled Escherichia coli, demonstrated that peritoneal macrophages from aminated glucan-treated rats had an enhanced ability to engulf and degrade bacteria. Scanning electron microscopy showed that macrophages from aminated glucan-treated animals were highly spread with prominent ruffling and bacteria located intracellularly, as opposed to macrophages from controls which were rounded with bacteria on the cell surface.
分别通过盲肠穿孔和回肠结扎诱导大鼠急性和亚急性腹膜炎，研究预防性腹腔内注射胺化葡聚糖对大鼠存活率以及粘连和脓肿形成的影响。在两种形式的腹膜炎中均发现死亡率显著降低。胺化葡聚糖预处理还显著减少了脓肿数量和腹膜粘连。与对照组相比，胺化葡聚糖处理的大鼠腹膜巨噬细胞数量增加了约三倍。使用32P标记的大肠杆菌进行的体外研究表明，胺化葡聚糖处理的大鼠的腹膜巨噬细胞吞噬和降解细菌的能力增强。扫描电子显微镜显示，胺化葡聚糖处理动物的巨噬细胞高度铺展，有明显的褶皱，细菌位于细胞内，而对照组的巨噬细胞呈圆形，细菌位于细胞表面。
