一种通过MK1从甘油连续生产赤藓糖醇的有效方法。
An Effective Method of Continuous Production of Erythritol from Glycerol by MK1.
作者信息
Rakicka Magdalena, Mirończuk Aleksandra M, Tomaszewska-Hetman Ludwika, Rywińska Anita, Rymowicz Waldemar
机构信息
Department of Biotechnology and Food Microbiology, Wroclaw University of Environmental and Life Sciences, Chełmońskiego St. 37, PL-51-630 Wroclaw, Poland.
出版信息
Food Technol Biotechnol. 2017 Mar;55(1):125-130. doi: 10.17113/ftb.55.01.17.4812.
Abstract
This study demonstrates the potential applicability of the UV mutant MK1 for the valorisation of glycerol and erythritol production in a chemostat culture. The aim of this research is to investigate the optimal C:N ratio in the feeding medium in order to enhance erythritol production. The highest erythritol concentration, at 113.1 g/L with a volumetric erythritol production rate of 1.1 g/(L·h) and a yield of 0.57 g/g, was obtained in the feeding medium with a C:N ratio of 80:1. Moreover, no residual glycerol was observed in the culture broth during cultivation. The chemical composition of the biomass was analysed. The contents of lysine and threonine in the biomass protein amino acid profile were higher than those required by the FAO/WHO for fodder yeast.
摘要
本研究证明了紫外线突变体MK1在恒化器培养中用于甘油增值和赤藓糖醇生产的潜在适用性。本研究的目的是研究进料培养基中的最佳碳氮比，以提高赤藓糖醇的产量。在碳氮比为80:1的进料培养基中，获得了最高的赤藓糖醇浓度，为113.1 g/L，体积赤藓糖醇生产率为1.1 g/(L·h)，产量为0.57 g/g。此外，在培养过程中，培养液中未观察到残留甘油。分析了生物质的化学成分。生物质蛋白质氨基酸谱中的赖氨酸和苏氨酸含量高于粮农组织/世界卫生组织对饲料酵母的要求。
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