Douglas J T, Palmer D A
Department of Microbiology, University of Hawaii, Honolulu 96822.
J Clin Microbiol. 1988 Jul;26(7):1353-6. doi: 10.1128/jcm.26.7.1353-1356.1988.
The smooth types of Brucella species express two lipopolysaccharides (LPSs) (A and M) which are antigenically distinct. Their existence as cross-reactive antigenic complexes makes definitive serology difficult. Murine hybridomas were produced and selected for their ability to produce monoclonal antibodies to the specific A- or M-LPS epitopes. The specificity of the monoclonal antibodies was determined by microplate enzyme-linked immunoassay, binding inhibition assay, microplate agglutination, and dot blot assay. Monoclonal antibody 12AE6 was specific for an epitope on the A LPS of Brucella spp., which was also expressed on Yersinia enterocolitica O:9. A unique epitope of M LPS was detected by monoclonal antibody 33.1.5. The two monoclonal antibodies did not exhibit cross-reactions when assayed with whole Brucella cells or purified M- and A-LPS preparations. Cross-reactive serology with polyvalent sera can be attributed to the presence of common antigenic determinants on both molecules. The use of A- and M-LPS-specific monoclonal antibodies has the potential to replace the more laborious methods involved in the production of monospecific sera.
布鲁氏菌属的光滑型菌株表达两种抗原性不同的脂多糖（LPS）（A和M）。它们作为交叉反应性抗原复合物的存在使得确切的血清学检测变得困难。制备了鼠杂交瘤，并根据其产生针对特定A或M-LPS表位的单克隆抗体的能力进行筛选。通过微孔板酶联免疫测定、结合抑制测定、微孔板凝集和斑点印迹测定来确定单克隆抗体的特异性。单克隆抗体12AE6对布鲁氏菌属A LPS上的一个表位具有特异性，该表位也在小肠结肠炎耶尔森菌O:9上表达。单克隆抗体33.1.5检测到M LPS的一个独特表位。当用完整的布鲁氏菌细胞或纯化的M-和A-LPS制剂进行检测时，这两种单克隆抗体未表现出交叉反应。多价血清的交叉反应血清学可归因于两种分子上存在共同的抗原决定簇。使用A-和M-LPS特异性单克隆抗体有可能取代生产单特异性血清所涉及的更费力的方法。
