Tsuruo T, Oh-Hara T, Yamori T, Tsukagoshi S, Ishikawa T, Sugano H
Division of Experimental Chemotherapy, Japanese Foundation for Cancer Research, Tokyo.
Clin Exp Metastasis. 1988 Mar-Apr;6(2):141-52. doi: 10.1007/BF01784845.
A metastatic tumor population was isolated in BALB/c mice during routine s.c. passage of the colon 26 adenocarcinoma. The tumor metastasized to lymph nodes, liver, spleen, ovary and kidney. A primary culture established from the s.c. growing tumor was composed of both adherent and nonadherent cells. These two cell types were successfully separated from the primary culture and designated CMS (suspension cells) and CMA (adherent cells). The CMS and CMA cell lines are morphologically distinct in culture; however both formed similar histopathologic tumors when inoculated s.c. Furthermore, both tumor lines showed identical metastatic patterns in BALB/c mice with involvement of lymph node, liver, spleen, ovary and kidney. CMS and CMA expressed T-antigen as revealed by FITC-labeled-anti-Thy 1.2 antibody. Chromosome analysis and morphologic studies by light and electron microscopy indicated that the present metastatic lines have no relationship with the colon 26 adenocarcinoma and seem to be non-thymic T-cell lymphosarcomas which developed spontaneously in BALB/c mice.
在结肠26腺癌的常规皮下传代过程中，从BALB/c小鼠中分离出一个转移性肿瘤群体。该肿瘤转移至淋巴结、肝脏、脾脏、卵巢和肾脏。从皮下生长的肿瘤建立的原代培养物由贴壁细胞和非贴壁细胞组成。这两种细胞类型成功地从原代培养物中分离出来，并分别命名为CMS（悬浮细胞）和CMA（贴壁细胞）。CMS和CMA细胞系在培养中形态不同；然而，当皮下接种时，两者都形成了相似的组织病理学肿瘤。此外，在BALB/c小鼠中，这两种肿瘤系都表现出相同的转移模式，累及淋巴结、肝脏、脾脏、卵巢和肾脏。如FITC标记的抗Thy 1.2抗体所示，CMS和CMA表达T抗原。染色体分析以及光镜和电镜形态学研究表明，目前的转移系与结肠26腺癌无关，似乎是在BALB/c小鼠中自发发生的非胸腺T细胞淋巴肉瘤。
