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A fast and sensitive method for detecting specific viral RNA in mammalian cells.

作者信息

Paeratakul U, De Stasio P R, Taylor M W

机构信息

Department of Biology, Indiana University, Bloomington 47405.

出版信息

J Virol. 1988 Apr;62(4):1132-5. doi: 10.1128/JVI.62.4.1132-1135.1988.

DOI:10.1128/JVI.62.4.1132-1135.1988
PMID:2450208
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC253120/
Abstract

A quick and sensitive method to quantitate viral RNA synthesis has been developed. Utilizing glutaraldehyde to fix infected cells onto nitrocellulose paper, viral RNA can be probed directly in situ. Viral message can be detected from as few as 10(4) infected cells. This technique can be used to study viral gene expression and can be adapted to screen the activity of antiviral agents such as interferon.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/620f/253120/bd45170ad378/jvirol00083-0050-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/620f/253120/4d901a62d606/jvirol00083-0049-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/620f/253120/ced2c04bb987/jvirol00083-0050-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/620f/253120/9665786beec3/jvirol00083-0050-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/620f/253120/bd45170ad378/jvirol00083-0050-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/620f/253120/4d901a62d606/jvirol00083-0049-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/620f/253120/ced2c04bb987/jvirol00083-0050-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/620f/253120/9665786beec3/jvirol00083-0050-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/620f/253120/bd45170ad378/jvirol00083-0050-c.jpg

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本文引用的文献

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Accumulation of histone repeat transcripts in the sea urchin egg pronucleus.组蛋白重复转录本在海胆卵原核中的积累。
Cell. 1981 May;24(2):385-91. doi: 10.1016/0092-8674(81)90328-7.
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Quick-blot: selective mRNA or DNA immobilization from whole cells.快速印迹法：从全细胞中选择性固定mRNA或DNA。
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The nucleotide and deduced amino acid sequences of the encephalomyocarditis viral polyprotein coding region.脑心肌炎病毒多聚蛋白编码区的核苷酸序列及推导的氨基酸序列。
Selected mutations of the duck hepatitis B virus P gene RNase H domain affect both RNA packaging and priming of minus-strand DNA synthesis.
鸭乙型肝炎病毒P基因核糖核酸酶H结构域的特定突变会影响RNA包装和负链DNA合成的引发。
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Expression of nonstructural protein NS3 of African horsesickness virus (AHSV): evidence for a cytotoxic effect of NS3 in insect cells, and characterization of the gene products in AHSV infected Vero cells.非洲马瘟病毒（AHSV）非结构蛋白NS3的表达：NS3在昆虫细胞中的细胞毒性作用证据以及AHSV感染的Vero细胞中基因产物的特性分析
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Interferon gamma-resistant mutants are defective in the induction of indoleamine 2,3-dioxygenase.γ-干扰素抗性突变体在吲哚胺2,3-双加氧酶的诱导方面存在缺陷。
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E1a-dependent expression of adenovirus genes in OTF963 embryonal carcinoma cells: role of E1a-induced differentiation.腺病毒基因在OTF963胚胎癌细胞中E1a依赖性表达：E1a诱导分化的作用
Proc Natl Acad Sci U S A. 1990 Oct;87(20):8041-5. doi: 10.1073/pnas.87.20.8041.
7
6-(3,4-Dichlorophenoxy)-3-(ethylthio)-2-pyridincarbonitrile inhibits poliovirus uncoating.6-(3,4-二氯苯氧基)-3-(乙硫基)-2-吡啶甲腈抑制脊髓灰质炎病毒脱壳。
Antimicrob Agents Chemother. 1990 Jun;34(6):1259-61. doi: 10.1128/AAC.34.6.1259.
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Detection of bovine viral diarrhea virus genome in leukocytes from persistently infected cattle by RNA-cDNA hybridization.通过RNA - cDNA杂交检测持续感染牛白细胞中的牛病毒性腹泻病毒基因组。
Can J Vet Res. 1990 Apr;54(2):256-9.
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Specific effect of interferon on the herpes simplex virus type 1 transactivation event.干扰素对单纯疱疹病毒1型反式激活事件的特异性作用。
J Virol. 1990 Jun;64(6):2588-93. doi: 10.1128/JVI.64.6.2588-2593.1990.
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J Virol. 1990 Sep;64(9):4329-37. doi: 10.1128/JVI.64.9.4329-4337.1990.
Nucleic Acids Res. 1984 Mar 26;12(6):2969-85. doi: 10.1093/nar/12.6.2969.
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Carcinogen-mediated induction of SV40 DNA synthesis in SV40 transformed Chinese hamster embryo cells.致癌物介导的SV40转化的中国仓鼠胚胎细胞中SV40 DNA合成的诱导。
Carcinogenesis. 1981;2(5):417-23. doi: 10.1093/carcin/2.5.417.
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Mengovirus RNA synthesis in productive and restrictive cell lines.
Virology. 1970 Sep;42(1):78-86. doi: 10.1016/0042-6822(70)90240-0.
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Anal Biochem. 1987 Apr;162(1):156-9. doi: 10.1006/abio.1987.9999.
9
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J Histochem Cytochem. 1978 Aug;26(8):677-9. doi: 10.1177/26.8.99471.