Bovetti Serena, Moretti Claudio, Fellin Tommaso
Department of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, 16163, Genova, Italy.
Microsc Res Tech. 2014 Jul;77(7):492-501. doi: 10.1002/jemt.22342. Epub 2014 Feb 7.
Mapping the activity of neuronal circuits with high resolution in the intact brain is a fundamental step toward understanding brain function. In the last several years, nonlinear microscopy combined with fluorescent activity reporters has become a crucial tool for achieving this goal. In this review article, we will highlight the principles underlying nonlinear microscopy and discuss its application to neuroscience, focusing on recent functional studies in the rodent neocortex in combination with genetically encoded calcium indicators.
在完整大脑中以高分辨率绘制神经回路的活动图是理解脑功能的基本步骤。在过去几年中，非线性显微镜与荧光活动报告分子相结合已成为实现这一目标的关键工具。在这篇综述文章中，我们将重点介绍非线性显微镜的基本原理，并讨论其在神经科学中的应用，尤其关注其与基因编码钙指示剂相结合在啮齿动物新皮层中的最新功能研究。
