Division of Urology, Department of Surgery, McGill University, Montreal, QC, Canada.
Andrology. 2014 May;2(3):322-5. doi: 10.1111/j.2047-2927.2014.00193.x. Epub 2014 Feb 11.
Over the past 20 years, numerous new methods have been developed to identify changes in the organization and composition of sperm chromatin as well as to determine the extent of DNA damage in the nuclei of spermatozoa. Although these methods are being used effectively in assessing how toxicants act on sperm chromatin quality in agricultural settings, their use as complementary biomarkers of sperm quality in assessing male fertility remains controversial. We review some key aspects of the assessment of sperm chromatin quality and DNA damage and identify some of the most widely used tests to monitor these endpoints. An approach to validate three tests by standardizing methodology and determining interlaboratory variation for each test using a standard set of samples is outlined.
在过去的 20 年中，已经开发出许多新的方法来识别精子染色质的组织和组成的变化，并确定精子核中 DNA 损伤的程度。尽管这些方法在评估农业环境中有毒物质对精子染色质质量的作用方面得到了有效应用，但它们作为评估男性生育能力的精子质量补充生物标志物的应用仍存在争议。我们回顾了评估精子染色质质量和 DNA 损伤的一些关键方面，并确定了一些最广泛使用的测试方法来监测这些终点。概述了一种通过标准化方法并使用标准样本集确定每个测试的实验室间变异性来验证三个测试的方法。
