Bassiri Farzaneh, Tavalaee Marziyeh, Nasr Esfahani Mohammad Hossein
Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran ; Isfahan Fertility and Infertility Center, Isfahan, Iran ; Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
Int J Fertil Steril. 2013 Oct;7(3):193-8. Epub 2013 Sep 18.
Sperm membrane integrity is not only important as a barrier between intra- and extra-cellular spaces, but also it can be considered as a sign of DNA integrity. Hypoosmotic swelling test reflects membrane integrity and has been used to evaluate sperm quality. Intracytoplasmic sperm injection (ICSI) in adjunct with hypo-osmotic swelling test (HOST) has been used for treatment of males with asthenozoospermia. Therefore, this study aims to evaluate correlation of different pattern of HOST with sperm parameters, protamine deficiency and apoptosis.
In this case-control study, sixteen semen samples were randomly collected from infertile normozospermic men. Semen samples were divided into two portions as follows: one portion was assessed for sperm parameters according toWorld Health Organization (WHO)-2010, while the other portion, after applying HOST procedure, was used for assessment of sperm morphology, protamine deficiency and late or early apoptosis. Statistical analysis was carried out using the Statistical Package for the Social Studies (SPSS 11.5).
Our results showed that, the lowest odds ratio (OR) of abnormal sperm head morphology and abnormal acrosome was in d-sperm as compared to a-pattern or nonviable spermatozoa (p=0.00, p=0.01). In addition, a significant correlation was observed between d-sperm with sperm concentration and percentage of DNA damage (p=0.03 and p=0.04, respectively). A significant correlation was observed between percentage of sperm motility and DNA fragmentation (r=-0.56; p=0.01). Furthermore, significant correlations were observed between percentages of early apoptotic sperm with protamine deficiency and sperm concentration (p=0.009 and p=0.01, respectively).
Significant correlations exist between d-pattern and sperm DNA integrity. Semen samples with low sperm concentration have low percentage of d-sperm which are mature and intact sperms.
精子膜完整性不仅作为细胞内和细胞外空间之间的屏障很重要，而且可以被视为DNA完整性的标志。低渗肿胀试验反映膜完整性，并已用于评估精子质量。卵胞浆内单精子注射（ICSI）联合低渗肿胀试验（HOST）已用于治疗弱精子症男性。因此，本研究旨在评估HOST不同模式与精子参数、鱼精蛋白缺乏和细胞凋亡之间的相关性。
在这项病例对照研究中，从不育的正常精子男性中随机收集了16份精液样本。精液样本分为两部分如下：一部分根据世界卫生组织（WHO）-2010标准评估精子参数，而另一部分在进行HOST程序后，用于评估精子形态、鱼精蛋白缺乏和晚期或早期细胞凋亡。使用社会科学统计软件包（SPSS 11.5）进行统计分析。
我们的结果表明，与a型模式或无活力精子相比，d型精子的异常精子头部形态和异常顶体的最低优势比（OR）（p=0.00，p=0.01）。此外，观察到d型精子与精子浓度和DNA损伤百分比之间存在显著相关性（分别为p=0.03和p=0.04）。观察到精子活力百分比与DNA片段化之间存在显著相关性（r=-0.56；p=0.01）。此外，观察到早期凋亡精子百分比与鱼精蛋白缺乏和精子浓度之间存在显著相关性（分别为p=0.009和p=0.01）。
d型模式与精子DNA完整性之间存在显著相关性。精子浓度低的精液样本中成熟且完整的d型精子百分比低。
