在BD Max系统上通过实时聚合酶链反应和熔解曲线分析检测碳青霉烯酶。
Detection of carbapenemases by real-time PCR and melt curve analysis on the BD Max system.
作者信息
Hofko Marjeta, Mischnik Alexander, Kaase Martin, Zimmermann Stefan, Dalpke Alexander H
机构信息
Department of Infectious Diseases, Medical Microbiology and Hygiene, Heidelberg University Hospital, Heidelberg, Germany.
出版信息
J Clin Microbiol. 2014 May;52(5):1701-4. doi: 10.1128/JCM.00373-14. Epub 2014 Feb 12.
Abstract
We developed a multiplex SYBR green real-time PCR for the BD Max instrument (BD Diagnostics, Sparks, MD) to detect a panel of carbapenemases. The assay was evaluated with 152 consecutive isolates sent to the German National Reference Laboratory, and 65/65 of the carbapenemase-positive and 87/87 of the carbapenemase-negative strains were identified correctly.
摘要
我们开发了一种用于BD Max仪器（BD诊断公司，美国马里兰州斯帕克斯）的多重SYBR Green实时PCR检测方法，以检测一组碳青霉烯酶。该检测方法用连续送至德国国家参考实验室的152株分离株进行评估，碳青霉烯酶阳性菌株中的65/65株和碳青霉烯酶阴性菌株中的87/87株均被正确鉴定。
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