Tang Chuanhong, Tan Yi, Zhang Jingsong, Zhou Shuai, Honda Yoichi, Zhang Henan
National Engineering Research Center of Edible Fungi, Key Laboratory for the Utilization of Edible Fungi in Southern China, Ministry of Agriculture, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai Key Open Laboratory of Agricultural Genetic Breeding, Shanghai 201403, China.
Laboratory of Forest Biochemistry, Graduate School of Agriculture, Kyoto University, Kyoto 6068502, Japan.
Foods. 2023 Jan 19;12(3):465. doi: 10.3390/foods12030465.
The spore powder of () has been proven to have a variety of pharmacological activities, and it has become a new resource for the development of health products and pharmaceuticals. However, the scarcity of natural resources, strict growth conditions and difficulty in controlling the stable yield, and quality of different culture batches seriously limit the development and utilization of spore powder. In the present study, the strain with the highest spore powder yield, G0109, was selected as the original strain to generate mutants of using ultraviolet ray irradiation. A total of 165 mutagenic strains were obtained, and fifty-five strains were chosen for the cultivation test. Importantly, one mutagenic strain with high spore powder yield and strong resistance to undesired microorganisms was acquired and named strain UV119. More cultivations demonstrated that the fruiting body and basidiospore yields from UV119 were, respectively, 8.67% and 19.27% higher than those of the parent (G0109), and the basidiospore yield was 20.56% higher than that of the current main cultivar "Longzhi No.1". In conclusion, this study suggested that ultraviolet ray irradiation is an efficient and practical method for Ganoderma strain improvement and thus provided a basis for the development and application of spore production and outstanding contributions to the rapid development of the industry.
（）的孢子粉已被证明具有多种药理活性，它已成为开发保健品和药品的新资源。然而，自然资源的稀缺、严格的生长条件以及难以控制不同培养批次的稳定产量和质量，严重限制了孢子粉的开发和利用。在本研究中，选择孢子粉产量最高的菌株G0109作为原始菌株，通过紫外线照射产生（）的突变体。共获得165个诱变菌株，选择55个菌株进行培养试验。重要的是，获得了一株孢子粉产量高且对有害微生物抗性强的诱变菌株，并命名为UV119菌株。更多培养表明，UV119的子实体和担孢子产量分别比亲本（G0109）高8.67%和19.27%，担孢子产量比当前主栽品种“龙芝1号”高20.56%。总之，本研究表明紫外线照射是灵芝菌株改良的一种高效实用方法，从而为孢子生产的开发和应用提供了依据，并为（）产业的快速发展做出了突出贡献。
