Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON, L8N 3Z5, Canada.
Arch Microbiol. 2014 Apr;196(4):307-10. doi: 10.1007/s00203-014-0964-4. Epub 2014 Feb 18.
The presence of bona fide outer membranes in members of the class Negativicutes is anomalous as phylogenetic analyses place this class within the phylum Firmicutes. To explore the relationships of a representative member of Negativicutes, we have performed a whole proteome BLAST analysis of Acidaminococcus intestini, which indicates that a substantial proportion (7 %) of the A. intestini proteome is closely related to sequences from members of the phylum Proteobacteria. In addition, we have identified key proteins involved in outer membrane biogenesis in A. intestini. This work highlights the need for further studies to define the relationships and evolutionary history of the Negativicutes.
在类阴性菌成员中存在真正的外膜是异常的，因为系统发育分析将该类归入厚壁菌门。为了探索阴性菌代表成员的关系，我们对肠道产酸菌进行了全蛋白质组 BLAST 分析，结果表明，肠道产酸菌蛋白质组的很大一部分（7%）与厚壁菌门成员的序列密切相关。此外，我们还鉴定了肠道产酸菌中外膜生物发生的关键蛋白。这项工作强调需要进一步研究来定义阴性菌的关系和进化历史。
