The James Buchanan Brady Urological Institute & Department of Urology, Johns Hopkins Medical Institutions, Baltimore, MD, USA.
Nanomedicine (Lond). 2014 Dec;9(18):2729-43. doi: 10.2217/nnm.13.207.
To develop and apply a heat-responsive and secreted reporter assay for comparing cellular response to nanoparticle (NP)- and macroscopic-mediated sublethal hyperthermia.
MATERIALS & METHODS: Reporter cells were heated by water bath (macroscopic heating) or iron oxide NPs activated by alternating magnetic fields (nanoscopic heating). Cellular responses to these thermal stresses were measured in the conditioned media by secreted luciferase assay.
RESULTS & CONCLUSION: Reporter activity was responsive to macroscopic and nanoparticle heating and activity correlated with measured macroscopic thermal dose. Significant cellular responses were observed with NP heating under doses that were insufficient to measurably change the temperature of the system. Under these conditions, the reporter response correlated with proximity to cells loaded with heated nanoparticles. These results suggest that NP and macroscopic hyperthermia may be distinctive under conditions of mild hyperthermia.
开发并应用一种热响应型分泌报告检测方法，用于比较细胞对纳米颗粒（NP）和宏观介导的亚致死性热疗的反应。
通过水浴（宏观加热）或交变磁场激活的氧化铁 NPs（纳米级加热）对报告细胞进行加热。通过分泌型荧光素酶检测法在条件培养基中测量细胞对这些热应激的反应。
报告基因活性对宏观和纳米颗粒加热有响应，且活性与测量的宏观热剂量相关。在不足以显著改变系统温度的 NP 加热剂量下，可观察到明显的细胞反应。在这些条件下，报告基因的反应与靠近负载加热纳米颗粒的细胞相关。这些结果表明，在温和热疗条件下，NP 和宏观热疗可能具有不同的特性。
