Abadi Amin Talebi Bezmin, Mobarez Ashraf Mohhabati, Bonten Marc J M, Wagenaar Jaap A, Kusters Johannes G
Department of Medical Microbiology, University Medical Center Utrecht, Heidelberglaan 100, Utrecht 3584 CX, The Netherlands.
BMC Gastroenterol. 2014 Feb 19;14:33. doi: 10.1186/1471-230X-14-33.
Numerous proteins have been proposed as virulence factors for the gram negative gastric bacterium Helicobacter pylori but only for a few this has unequivocally been demonstrated. The aim of the current study was to evaluate the association of the putative virulence factors tnpA and tnpB (no cagA) with H. pylori associated gastroduodenal diseases.
A PCR based assay was used to determine the presence of the tnpA and tnpB genes, as well as of cagA, in 360H. pylori strains isolated from H. pylori infected patients.
Of 360 H. pylori culture positive patients (196 men, 164 women; average age 42.1 years (range 17-73), 95 had gastritis, 92 had gastric ulcers, 108 had duodenal ulcers, and 65 had gastric cancer. Using the gastritis group as a reference a significantly aberrant gene distribution was observed for the tnpA (Relative risk: 1.45; 95% CI 1.04-1.93), the cagA (Relative risk: 1.81; 95% CI 1.44-2.29), but not the tnpB gene in the gastric cancer group.
The increased incidence of the tnpA gene in gastric cancer patients suggests a role of the tnpA gene in the development of H. pylori induced gastric cancer.
许多蛋白质被认为是革兰氏阴性胃细菌幽门螺杆菌的毒力因子，但只有少数已得到明确证实。本研究的目的是评估假定的毒力因子tnpA和tnpB（无cagA）与幽门螺杆菌相关的胃十二指肠疾病之间的关联。
采用基于聚合酶链反应的检测方法，确定从幽门螺杆菌感染患者中分离出的360株幽门螺杆菌菌株中tnpA和tnpB基因以及cagA的存在情况。
在360例幽门螺杆菌培养阳性患者中（196例男性，164例女性；平均年龄42.1岁（范围17 - 73岁）），95例患有胃炎，92例患有胃溃疡，108例患有十二指肠溃疡，65例患有胃癌。以胃炎组作为参照，在胃癌组中观察到tnpA（相对风险：1.45；95%可信区间1.04 - 1.93）、cagA（相对风险：1.81；95%可信区间1.44 - 2.29）基因分布存在显著异常，但tnpB基因无此情况。
胃癌患者中tnpA基因发生率的增加表明tnpA基因在幽门螺杆菌诱导的胃癌发生过程中起作用。
