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从人类肠道微生物群中分离出的口腔短双歧杆菌菌株JMR01的基因组测序草图

Draft Genome Sequencing of Methanobrevibacter oralis Strain JMR01, Isolated from the Human Intestinal Microbiota.

作者信息

Khelaifia Saber, Garibal Marc, Robert Catherine, Raoult Didier, Drancourt Michel

机构信息

Aix Marseille Université, URMITE, UMR63 CNRS 7278, IRD 198, Inserm 1095, Marseille, France.

出版信息

Genome Announc. 2014 Feb 20;2(1):e00073-14. doi: 10.1128/genomeA.00073-14.

DOI:10.1128/genomeA.00073-14
PMID:24558239
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3931360/
Abstract

Methanobrevibacter oralis, an anaerobic methanogenic archaeon, has been previously isolated from the human oral cavity. Here, sequencing a stool isolate (strain JMR01) yielded a 2.065-Mb genome with a 27.78% G+C content containing a total of 2,042 open reading frames and 3 clusters of regularly interspaced short palindromic repeat (CRISPR) loci with associated Cas proteins.

摘要

口腔甲烷短杆菌是一种厌氧产甲烷古菌,此前已从人类口腔中分离出来。在这里,对一株粪便分离株(菌株JMR01)进行测序,得到了一个2.065兆碱基的基因组,其鸟嘌呤与胞嘧啶含量为27.78%,共包含2042个开放阅读框和3个成簇规律间隔短回文重复序列(CRISPR)位点以及相关的Cas蛋白。

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本文引用的文献

1
Draft Genome Sequence of a Human-Associated Isolate of Methanobrevibacter arboriphilicus, the Lowest-G+C-Content Archaeon.嗜树栖短柄产甲烷菌(Methanobrevibacter arboriphilicus)人源分离株的基因组序列草图,该古菌具有最低的G+C含量
Genome Announc. 2014 Jan 23;2(1):e01181-13. doi: 10.1128/genomeA.01181-13.
2
A versatile medium for cultivating methanogenic archaea.一种用于培养产甲烷古菌的多功能培养基。
PLoS One. 2013 Apr 17;8(4):e61563. doi: 10.1371/journal.pone.0061563. Print 2013.
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Real-time PCR quantification of Methanobrevibacter oralis in periodontitis.实时聚合酶链反应定量检测牙周炎中的产甲烷短杆菌。
J Clin Microbiol. 2013 Mar;51(3):993-4. doi: 10.1128/JCM.02863-12. Epub 2012 Dec 19.
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The RAST Server: rapid annotations using subsystems technology.RAST服务器:使用子系统技术进行快速注释。
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Genomic and metabolic adaptations of Methanobrevibacter smithii to the human gut.史氏甲烷短杆菌对人类肠道的基因组和代谢适应性
Proc Natl Acad Sci U S A. 2007 Jun 19;104(25):10643-8. doi: 10.1073/pnas.0704189104. Epub 2007 Jun 11.
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Methanogenic Archaea and human periodontal disease.产甲烷古菌与人类牙周病
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