Dianzani C, Foreman J C
Instituto di Farmacologia dell' Universita di Torino, Italy.
Agents Actions. 1988 Apr;23(3-4):214-6. doi: 10.1007/BF02142544.
Rat peritoneal mast cells were pretreated for 10 min at 37 degrees C with either substance P (SP, 3 or 6 microM) or compound 48/80 (37.5, 50 or 75 ng/ml). The effect of this pretreatment on the subsequent responsiveness of the cells to SP was studied. Both SP and compound 48/80 pretreatment of rat peritoneal mast cells inhibited the subsequent response of the cells to SP. The degree of inhibition produced by either SP or compound 48/80 was dependent on the concentration used to pretreat the cells. Inhibition of the response of the cells to SP was observed whether or not the pretreating agent was removed or remained in contact with the cells during the subsequent stimulation with SP. It is concluded that compound 48/80 and SP desensitize the cells to subsequent stimulation by SP and possible mechanisms for this are discussed.
将大鼠腹膜肥大细胞在37℃下用P物质（SP，3或6微摩尔）或化合物48/80（37.5、50或75纳克/毫升）预处理10分钟。研究了这种预处理对细胞随后对SP反应性的影响。大鼠腹膜肥大细胞的SP和化合物48/80预处理均抑制了细胞随后对SP的反应。SP或化合物48/80产生的抑制程度取决于用于预处理细胞的浓度。在随后用SP刺激期间，无论预处理剂是否被去除或仍与细胞接触，均观察到细胞对SP反应的抑制。得出结论：化合物48/80和SP使细胞对SP的后续刺激脱敏，并讨论了可能的机制。
