Pérez-Chaparro Paula Juliana, Cerdeira Louise Teixeira, Queiroz Maíse Gomes, de Lima Clayton Pereira Silva, Levy Carlos Emílio, Pavez Mónica, Lincopan Nilton, Gonçalves Evonnildo Costa, Mamizuka Elsa Masae, Sampaio Jorge Luiz Mello, Nunes Marcio Roberto Teixeira, McCulloch John Anthony
Centro de Inovações Tecnológicas do Instituto Evandro Chagas/SVS/Ministério da Saúde, Ananindeua, Pará, Brazil.
Antimicrob Agents Chemother. 2014 May;58(5):2958-60. doi: 10.1128/AAC.02341-13. Epub 2014 Feb 24.
We sequenced the oldest blaKPC-2-bearing plasmid isolated in Brazil and another plasmid also carried by a Klebsiella pneumoniae strain of sequence type 442 (ST442), isolated 52 months later. Both plasmids present an IncN backbone and few acquired regions. Because the 2005 plasmid presented deletions and a truncated gene within Tn4401b compared to the 2009 plasmid, we can thus infer that IncN blaKPC-2-bearing plasmids pFCF1305 and pFCF3SP had a common ancestor circulating in Brazil prior to May 2005.
我们对巴西分离出的携带blaKPC-2的最古老质粒以及52个月后从一株序列类型为442（ST442）的肺炎克雷伯菌菌株中分离出的另一质粒进行了测序。这两个质粒均具有IncN骨架和少量获得性区域。由于与2009年的质粒相比，2005年的质粒在Tn4401b内存在缺失和一个截短基因，因此我们可以推断，携带blaKPC-2的IncN质粒pFCF1305和pFCF3SP在2005年5月之前有一个共同的祖先在巴西传播。
