Zhao Yong, Wang Bao-en, Zhang Shu-wen, Yang Shu-min, Wang Hong, Ren Ai-min, Yi En-tong
Department of Infectious Diseases, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China.
Chin J Integr Med. 2015 Mar;21(3):211-6. doi: 10.1007/s11655-014-1805-7. Epub 2014 Feb 27.
To isolate antifungal compound from Paeonia suffruticosa, and to find the antifungal mechanisms by observing the ultrastructural modifications of yeasts in growth phase produced by 1,2,3,4,6-penta-O-galloyl-beta-D-glucose (PGG).
Peony (Paeonia suffruticosa) root bark (PRB) was separated by solvent extraction and purified by high performance liquid chromatography (HPLC) method using analytical and preparative reversed phase C18 column on the basis of bio-assay method. In order to investigate the antifungal mechanism of PGG, Yeasts were submitted to different concentrations [3 × minimum inhibition concentration (MIC), 0.3 × MIC] for 1 h under constant stirring at 30 °C, and transmission electron microscopy was performed.
Based on the antifungal activity of PRB on Candida glabrata CBS138, the antifungal compound were isolated in ethyl acetate layer of PRB and identified as PGG by mass spectrometry, 1H nuclear magnetic resonance (NMR) analyses, with molecular weight of 940 and molecular formular as C41H32O26. Transmission electron microscopy showed that PGG degraded the cell wall envelope.
The results suggest that PGG may be responsible for the antifungal activity of PRB by disrupting the structure of cell wall directly.
从牡丹中分离抗真菌化合物，并通过观察1,2,3,4,6 - 五 - O - 没食子酰基 - β - D - 葡萄糖（PGG）对生长期酵母超微结构的改变来探究其抗真菌机制。
采用溶剂萃取法分离牡丹（Paeonia suffruticosa）根皮（PRB），并在生物测定法的基础上，使用分析型和制备型反相C18柱通过高效液相色谱（HPLC）法进行纯化。为了研究PGG的抗真菌机制，将酵母在30℃持续搅拌下分别置于不同浓度[3×最低抑菌浓度（MIC），0.3×MIC]中处理1小时，然后进行透射电子显微镜观察。
基于PRB对光滑念珠菌CBS138的抗真菌活性，从PRB的乙酸乙酯层中分离出抗真菌化合物，并通过质谱、1H核磁共振（NMR）分析鉴定为PGG，其分子量为940，分子式为C41H32O26。透射电子显微镜显示PGG破坏了细胞壁包膜。
结果表明PGG可能通过直接破坏细胞壁结构而发挥PRB的抗真菌活性。
