a Beijing Key Laboratory of Bioprocess, College of Life Science and Technology , Beijing University of Chemical Technology , Beijing , P.R. China.
Crit Rev Food Sci Nutr. 2014;54(12):1548-61. doi: 10.1080/10408398.2011.642905.
Accompanied by industrial globalization, rapid urbanization, and population increment, mass production and staple trading for food consumption are upsoaring continuously, foodborne disease resulted from various food safety issues is currently a crucial public health concern worldwide, which has not only created a great burden on both economy and society, but also greatly threatened the sustainability of mankind's livelihood and human reproduction. In order to better ensure food safety and thus effectively curb the occurrence of foodborne diseases, the development and evolving of inspection strategies are indispensable measures for quality assurance and conformity assessment. Nowadays, as complementary measures to and with advantageous merits over classic analytical methods, highly specific and selective aptamer-based assays have found their increasingly important roles in various domains of food analysis. This critical review summarizes the advantages of aptamer as compared with antibody, introduces important evolving variants of systematic evolution of ligands by exponential enrichment (SELEX), and presents an overview of potential aptamer applications for food safety.
伴随着工业化全球化、城市化进程加快和人口增长,食品消费的大规模生产和主食贸易持续飙升,由各种食品安全问题引发的食源性疾病目前是全球范围内一个至关重要的公共卫生关注点,这不仅给经济和社会带来了巨大负担,也严重威胁到人类生计和人类繁衍的可持续性。为了更好地确保食品安全,从而有效遏制食源性疾病的发生,检测策略的发展和演变是质量保证和合格评定的必要措施。如今,作为经典分析方法的补充措施,并且具有优于经典分析方法的优势,基于高特异性和高选择性适配体的检测方法在食品分析的各个领域中发挥着越来越重要的作用。本文综述了适配体相对于抗体的优势,介绍了配体指数富集进化技术(SELEX)的重要演变变体,并概述了适配体在食品安全中的潜在应用。