Minnesota Veterinary Diagnostic Laboratory, St. Paul, Minnesota, United States of America ; University of Minnesota, Department of Veterinary Population Medicine, St. Paul, Minnesota, United States of America.
Minnesota Veterinary Diagnostic Laboratory, St. Paul, Minnesota, United States of America.
PLoS One. 2014 Feb 21;9(2):e87593. doi: 10.1371/journal.pone.0087593. eCollection 2014.
During both regulatory and routine surveillance sampling of baitfish from the states of Illinois, Minnesota, Montana, and Wisconsin, USA, isolates (n = 20) of a previously unknown picornavirus were obtained from kidney/spleen or entire viscera of fathead minnows (Pimephales promelas) and brassy minnows (Hybognathus hankinsoni). Following the appearance of a diffuse cytopathic effect, examination of cell culture supernatant by negative contrast electron microscopy revealed the presence of small, round virus particles (∼ 30-32 nm), with picornavirus-like morphology. Amplification and sequence analysis of viral RNA identified the agent as a novel member of the Picornaviridae family, tentatively named fathead minnow picornavirus (FHMPV). The full FHMPV genome consisted of 7834 nucleotides. Phylogenetic analysis based on 491 amino acid residues of the 3D gene showed 98.6% to 100% identity among the 20 isolates of FHMPV compared in this study while only 49.5% identity with its nearest neighbor, the bluegill picornavirus (BGPV) isolated from bluegill (Lepomis macrochirus). Based on complete polyprotein analysis, the FHMPV shared 58% (P1), 33% (P2) and 43% (P3) amino acid identities with BGPV and shared less than 40% amino acid identity with all other picornaviruses. Hence, we propose the creation of a new genus (Piscevirus) within the Picornaviridae family. The impact of FHMPV on the health of fish populations is unknown at present.
在美国伊利诺伊州、明尼苏达州、蒙大拿州和威斯康星州，在对诱饵鱼进行监管和常规监测抽样时，从胖头鱼（Pimephales promelas）和黄鲈（Hybognathus hankinsoni）的肾脏/脾脏或整个内脏中分离出了一种以前未知的小核糖核酸病毒（picornavirus）的分离株（n = 20）。在出现弥漫性细胞病变效应后，通过负染电子显微镜检查细胞培养上清液，发现存在小而圆的病毒颗粒（∼30-32nm），具有小核糖核酸病毒样形态。病毒 RNA 的扩增和序列分析鉴定该病原体是小核糖核酸病毒科的一个新成员，暂定名为胖头鱼小核糖核酸病毒（FHMPV）。FHMPV 的全基因组由 7834 个核苷酸组成。基于 3D 基因的 491 个氨基酸残基的系统发育分析表明，本研究中比较的 20 株 FHMPV 之间的同源性为 98.6%至 100%，而与最接近的邻居蓝鳃太阳鱼小核糖核酸病毒（BGPV）的同源性仅为 49.5%，BGPV 是从蓝鳃太阳鱼（Lepomis macrochirus）中分离出来的。基于完整的多蛋白分析，FHMPV 与 BGPV 的 P1、P2 和 P3 区域分别共享 58%、33%和 43%的氨基酸同一性，与其他小核糖核酸病毒的氨基酸同一性小于 40%。因此，我们建议在小核糖核酸病毒科内创建一个新属（Piscevirus）。目前尚不清楚 FHMPV 对鱼类种群健康的影响。
