Department of Pharmacology and Pharmaceutical Sciences, University of Southern California, School of Pharmacy , Los Angeles, California 90089, United States.
Org Lett. 2014 Mar 21;16(6):1676-9. doi: 10.1021/ol5003384. Epub 2014 Mar 5.
A facile genetic methodology in the filamentous fungus Aspergillus nidulans allowed exchange of the starter unit ACP transacylase (SAT) domain in the nonreduced polyketide synthase (NR-PKS) AfoE of the asperfuranone pathway with the SAT domains from 10 other NR-PKSs. The newly created hybrid with the NR-PKS AN3386 is able to accept a longer starter unit in place of the native substrate to create a novel aromatic polyketide in vivo.
一种在丝状真菌构巢曲霉中简便的遗传方法，使得 Asperfuranone 途径中的非还原型聚酮合酶（NR-PKS）AfoE 的起始单元 ACP 转酰基酶（SAT）结构域可以与其他 10 种 NR-PKS 的 SAT 结构域进行交换。与 NR-PKS AN3386 新创建的杂种能够接受更长的起始单元代替天然底物，从而在体内生成一种新型芳香族聚酮。
