• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

M2 delta, a candidate for the structure lining the ionic channel of the nicotinic cholinergic receptor.

作者信息

Oiki S, Danho W, Madison V, Montal M

机构信息

Roche Institute of Molecular Biology, Nutley, NJ 07110.

出版信息

Proc Natl Acad Sci U S A. 1988 Nov;85(22):8703-7. doi: 10.1073/pnas.85.22.8703.

DOI:10.1073/pnas.85.22.8703
PMID:2460876
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC282529/
Abstract

A synthetic 23-mer peptide that mimics the sequence of the putative transmembrane M2 segment of the Torpedo californica acetylcholine receptor (AcChoR) delta subunit--Glu-Lys-Met-Ser-Thr-Ala-Ile-Ser-Val-Leu-Leu-Ala-Gln-Ala-Val-Phe-Leu- Leu-Leu-Thr-Ser-Gln-Arg--forms discrete ionic channels in phosphatidylcholine bilayers. In contrast, a synthetic peptide that mimics the sequence of the putative M1 transmembrane segment of the Torpedo AcChoR delta subunit--Leu-Phe-Tyr-Val-Ile-Asn-Phe-Ile-Thr-Pro-Cys-Val-Leu-Ile-Ser-Phe- Leu-Ala-Ser-Leu-Ala-Phe-Tyr--does not form channels. The synthetic M2 delta channel peptide exhibits features that are characteristic of the authentic AcChoR channel, such as single channel conductances, discrimination of cations over anions, and channel lifetimes for open and closed states in the millisecond time range. Energetic considerations suggest that an aggregate of five amphipathic alpha-helices conforms the channel. Thus, the M2 segment may be a component of the AcChoR channel structure.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a344/282529/d60396e6330c/pnas00301-0343-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a344/282529/d60396e6330c/pnas00301-0343-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a344/282529/d60396e6330c/pnas00301-0343-a.jpg

相似文献

1
M2 delta, a candidate for the structure lining the ionic channel of the nicotinic cholinergic receptor.
Proc Natl Acad Sci U S A. 1988 Nov;85(22):8703-7. doi: 10.1073/pnas.85.22.8703.
2
Synporins--synthetic proteins that emulate the pore structure of biological ionic channels.合成孔蛋白——模拟生物离子通道孔结构的合成蛋白质。
Proc Natl Acad Sci U S A. 1990 Sep;87(18):6929-33. doi: 10.1073/pnas.87.18.6929.
3
The human erythrocyte anion-transport protein. Partial amino acid sequence, conformation and a possible molecular mechanism for anion exchange.人类红细胞阴离子转运蛋白。部分氨基酸序列、构象及阴离子交换的一种可能分子机制。
Biochem J. 1983 Sep 1;213(3):577-86. doi: 10.1042/bj2130577.
4
Amino acid sequence of a ferredoxin from Bumilleriopsis filiformis, a yellow-green alga: relationship with red algae, protoflorideophyceae, and filamentous blue-green algae.丝状黄群藻（一种黄绿藻）中铁氧化还原蛋白的氨基酸序列：与红藻、原红藻纲和丝状蓝藻的关系
J Biochem. 1983 Nov;94(5):1451-5.
5
Synthetic peptides and four-helix bundle proteins as model systems for the pore-forming structure of channel proteins. I. Transmembrane segment M2 of the nicotinic cholinergic receptor channel is a key pore-lining structure.合成肽和四螺旋束蛋白作为通道蛋白成孔结构的模型系统。I. 烟碱型胆碱能受体通道的跨膜片段M2是关键的孔内衬结构。
J Biol Chem. 1993 Jul 15;268(20):14601-7.
6
Complete amino acid sequence of murine beta 2-microglobulin: structural evidence for strain-related polymorphism.小鼠β2-微球蛋白的完整氨基酸序列：品系相关多态性的结构证据。
Proc Natl Acad Sci U S A. 1981 Jan;78(1):554-8. doi: 10.1073/pnas.78.1.554.
7
An electrophysiological and spectroscopic study of the properties and structure of biological calcium channels. Investigations of a model ion channel.
Biochim Biophys Acta. 1992 May 21;1106(2):264-72. doi: 10.1016/0005-2736(92)90005-7.
8
Region of peptide 125-147 of acetylcholine receptor alpha subunit is exposed at neuromuscular junction and induces experimental autoimmune myasthenia gravis, T-cell immunity, and modulating autoantibodies.乙酰胆碱受体α亚基125 - 147肽段区域在神经肌肉接头处暴露，并诱导实验性自身免疫性重症肌无力、T细胞免疫及调节自身抗体。
Proc Natl Acad Sci U S A. 1985 Dec;82(24):8805-9. doi: 10.1073/pnas.82.24.8805.
9
Purification and structural characterization of insulin and glucagon from the bichir Polypterus senegalis (Actinopterygii: Polypteriformes).来自塞内加尔多鳍鱼（辐鳍鱼纲：多鳍鱼目）的胰岛素和胰高血糖素的纯化及结构表征
Gen Comp Endocrinol. 1998 Jan;109(1):86-93. doi: 10.1006/gcen.1997.7007.
10
Interaction of acetylcholinesterase with the G4 domain of the laminin alpha1-chain.乙酰胆碱酯酶与层粘连蛋白α1链的G4结构域的相互作用。
Biochem J. 2008 May 1;411(3):507-14. doi: 10.1042/BJ20071404.

引用本文的文献

1
Asymmetric Lipid Bilayers and Potassium Channels Embedded Therein in the Contact Bubble Bilayer.接触小泡脂双层中的不对称脂双层和嵌入其中的钾通道。
Methods Mol Biol. 2024;2796:1-21. doi: 10.1007/978-1-0716-3818-7_1.
2
Systematic Design and Validation of Ion Channel Stabilization of Amphipathic α-Helical Peptides Incorporating Tryptophan Residues.含色氨酸残基的两亲性α-螺旋肽离子通道稳定化的系统设计与验证
ACS Omega. 2020 Dec 29;6(1):723-732. doi: 10.1021/acsomega.0c05254. eCollection 2021 Jan 12.
3
Channel function reconstitution and re-animation: a single-channel strategy in the postcrystal age.

本文引用的文献

1
Helix to helix packing in proteins.蛋白质中的螺旋-螺旋堆积
J Mol Biol. 1981 Jan 5;145(1):215-50. doi: 10.1016/0022-2836(81)90341-7.
2
Primary structures of beta- and delta-subunit precursors of Torpedo californica acetylcholine receptor deduced from cDNA sequences.从cDNA序列推导的加州电鳐乙酰胆碱受体β和δ亚基前体的一级结构。
Nature. 1983 Jan 20;301(5897):251-5. doi: 10.1038/301251a0.
3
Nucleotide and deduced amino acid sequences of Torpedo californica acetylcholine receptor gamma subunit.加州电鳐乙酰胆碱受体γ亚基的核苷酸及推导的氨基酸序列
通道功能的重构与复活：后晶体时代的单通道策略
J Physiol. 2015 Jun 15;593(12):2553-73. doi: 10.1113/JP270025. Epub 2015 May 14.
4
Molecular dynamics simulations of homo-oligomeric bundles embedded within a lipid bilayer.嵌入双层脂膜中的同源寡聚束的分子动力学模拟。
Biophys J. 2013 Oct 1;105(7):1569-80. doi: 10.1016/j.bpj.2013.07.053.
5
Coarse-grained molecular dynamics of tetrameric transmembrane peptide bundles within a lipid bilayer.脂质双层内四聚体跨膜肽束的粗粒度分子动力学
Chem Phys Lipids. 2010 Jun;163(6):530-7. doi: 10.1016/j.chemphyslip.2010.04.007. Epub 2010 Apr 28.
6
Expression of the novel wheat gene TM20 confers enhanced cadmium tolerance to bakers' yeast.新型小麦基因TM20的表达赋予面包酵母更强的镉耐受性。
J Biol Chem. 2008 Jun 6;283(23):15893-902. doi: 10.1074/jbc.M708947200. Epub 2008 Apr 14.
7
Using ion channel-forming peptides to quantify protein-ligand interactions.利用形成离子通道的肽来量化蛋白质-配体相互作用。
J Am Chem Soc. 2008 Jan 30;130(4):1453-65. doi: 10.1021/ja077555f. Epub 2008 Jan 8.
8
Conformation and environment of channel-forming peptides: a simulation study.形成通道的肽的构象与环境：一项模拟研究。
Biophys J. 2006 Mar 15;90(6):1855-64. doi: 10.1529/biophysj.105.069625. Epub 2005 Dec 30.
9
Molecular dynamics simulation of the M2 helices within the nicotinic acetylcholine receptor transmembrane domain: structure and collective motions.烟碱型乙酰胆碱受体跨膜结构域内M2螺旋的分子动力学模拟：结构与集体运动
Biophys J. 2005 May;88(5):3321-33. doi: 10.1529/biophysj.104.052878. Epub 2005 Feb 18.
10
The transmembrane domain of the acetylcholine receptor: insights from simulations on synthetic peptide models.乙酰胆碱受体的跨膜结构域：基于合成肽模型模拟的见解
Biophys J. 2005 Feb;88(2):959-70. doi: 10.1529/biophysj.104.049726. Epub 2004 Nov 19.
Proc Natl Acad Sci U S A. 1983 Feb;80(4):1111-5. doi: 10.1073/pnas.80.4.1111.
4
Complete mRNA coding sequence of the acetylcholine binding alpha-subunit of Torpedo marmorata acetylcholine receptor: a model for the transmembrane organization of the polypeptide chain.电鳐乙酰胆碱受体乙酰胆碱结合α亚基的完整mRNA编码序列：多肽链跨膜组织的模型
Proc Natl Acad Sci U S A. 1983 Apr;80(7):2067-71. doi: 10.1073/pnas.80.7.2067.
5
Analysis of membrane and surface protein sequences with the hydrophobic moment plot.利用疏水矩图分析膜蛋白和表面蛋白序列。
J Mol Biol. 1984 Oct 15;179(1):125-42. doi: 10.1016/0022-2836(84)90309-7.
6
A structural model of the acetylcholine receptor channel based on partition energy and helix packing calculations.基于分配能量和螺旋堆积计算的乙酰胆碱受体通道结构模型。
Biophys J. 1984 Jan;45(1):249-61. doi: 10.1016/S0006-3495(84)84152-1.
7
Amphipathic analysis and possible formation of the ion channel in an acetylcholine receptor.乙酰胆碱受体的两亲性分析及离子通道的可能形成
Proc Natl Acad Sci U S A. 1984 Jan;81(1):155-9. doi: 10.1073/pnas.81.1.155.
8
Acetylcholine receptor channel ionic selectivity: ions experience an aqueous environment.乙酰胆碱受体通道的离子选择性：离子处于水性环境中。
Proc Natl Acad Sci U S A. 1983 Oct;80(19):6110-3. doi: 10.1073/pnas.80.19.6110.
9
Structural and functional diversity in 4-alpha-helical proteins.4-α-螺旋蛋白中的结构与功能多样性。
Nature. 1980 Sep 4;287(5777):82-4. doi: 10.1038/287082a0.
10
The permeability of endplate channels to monovalent and divalent metal cations.终板通道对单价和二价金属阳离子的通透性。
J Gen Physiol. 1980 May;75(5):493-510. doi: 10.1085/jgp.75.5.493.