Saatli Bahadir, Kizildag Sefa, Cagliyan Erkan, Dogan Erbil, Saygili Ugur
Department of Obstetrics and Gynecology, School of Medicine, Dokuz Eylul University, 35340, Inciralti, Izmir, Turkey,
Int Urogynecol J. 2014 Jul;25(7):971-7. doi: 10.1007/s00192-014-2347-4. Epub 2014 Mar 11.
We aimed to compare expression levels of antiapoptotic and proapoptotic genes in parametrial and vaginal tissues from postmenopausal women with and without pelvic organ prolapse (POP). We hypothesized that the expression of genes that induce apoptosis may be altered in vaginal and parametrial tissues in postmenopausal women with POP.
Samples of vaginal and parametrial tissues were obtained from postmenopausal women with (n = 10) and without (n = 10) POP who underwent vaginal or abdominal hysterectomy. Expression levels of antiapoptotic (BCL-2, BCL-XL) and proapoptotic (BAX, BAD) genes were studied by real-time reverse-transcription polymerase chain reaction (RT-PCR).
Gene expression levels of BCL-2 (P < 0.001), BCL-XL (P < 0.001), BAX (p = 0.001), and BAD (p = 0.004) were all higher in vaginal tissues from the POP group compared with the non-POP group. Similarly, gene expression levels of BCL-2 (p < 0.001), BCL-XL (p < 0.001), BAX (p < 0.001), and BAD (p < 0.001) in parametrial tissues were also significantly higher in the POP group compared with the non-POP group. Additionally, expression levels of BCL-2 (p = 0.05), BCL-XL (p < 0.05), BAX (p = 0.05), and BAD (p = 0.07) in the POP group were higher in parametrial tissue than in vaginal tissue samples.
Antiapoptotic and proapoptotic gene expression levels differed significantly between postmenopausal women with and without POP. Bcl-2 family genes were overexpressed in the parametrium of patients with POP compared with vaginal tissue, suggesting that the processes responsible for POP have a greater effect on parametrial tissue than vaginal tissue during the development of POP.
我们旨在比较有和没有盆腔器官脱垂（POP）的绝经后女性的宫旁组织和阴道组织中抗凋亡基因和促凋亡基因的表达水平。我们假设，在患有POP的绝经后女性的阴道和宫旁组织中，诱导凋亡的基因表达可能会发生改变。
从接受阴道或腹部子宫切除术的有（n = 10）和没有（n = 10）POP的绝经后女性中获取阴道和宫旁组织样本。通过实时逆转录聚合酶链反应（RT-PCR）研究抗凋亡（BCL-2、BCL-XL）和促凋亡（BAX、BAD）基因的表达水平。
与非POP组相比，POP组阴道组织中BCL-2（P < 0.001）、BCL-XL（P < 0.001）、BAX（p = 0.001）和BAD（p = 0.004）的基因表达水平均更高。同样，与非POP组相比，POP组宫旁组织中BCL-2（p < 0.001）、BCL-XL（p < 0.001）、BAX（p < 0.001）和BAD（p < 0.001）的基因表达水平也显著更高。此外，POP组宫旁组织中BCL-2（p = 0.05）、BCL-XL（p < 0.05）、BAX（p = 0.05）和BAD（p = 0.07）的表达水平高于阴道组织样本。
有和没有POP的绝经后女性之间抗凋亡和促凋亡基因表达水平存在显著差异。与阴道组织相比，POP患者宫旁组织中Bcl-2家族基因过表达，这表明在POP发生过程中，导致POP的过程对宫旁组织的影响大于阴道组织。
