Physiology Division, Zoology Department, Faculty of Science, Mansoura University, Mansoura, Egypt.
Cytotechnology. 2015 May;67(3):437-47. doi: 10.1007/s10616-014-9702-7. Epub 2014 Mar 11.
Nitrate has described as an endocrine disruptor that promotes onset of diabetes. This study was undertaken to evaluate diabetic effect of high nitrate intake in young and adult male rats and its amelioration by fenugreek administration. The study revealed significant increase in serum glucose and blood glycosylated hemoglobin (HbA1c%), while serum insulin and liver glycogen were decreased among nitrate exposed animals, in particular the young group. A significant reduction in the body weight gain and serum thyroid hormones (T4 & T3) was also recorded. Further reduction in serum levels of urea and creatinine, as well as total protein in serum, liver and pancreas was demonstrated, with elevation in their levels in the urine of all nitrate exposed groups. Meanwhile, the activity of serum transaminases (ALT and AST) was increased, with decline in their activity in the liver tissue. In addition, an elevation in serum total bilirubin, tissues (liver and pancreas) nitric oxide and lipid profile, as well as liver activity of glucose-6-phosphatase was recorded. Fenugreek administration to nitrate exposed rats was found to be effective in alleviating hyperglycemia and other biochemical changes characterizing nitrate-induced diabetes. So, fenugreek can be considered to possess potent activity against onset of nitrate induced-diabetes.
硝酸盐被描述为一种内分泌干扰物，可促进糖尿病的发生。本研究旨在评估高硝酸盐摄入对年轻和成年雄性大鼠的糖尿病影响及其通过摄入胡芦巴的改善作用。研究表明，暴露于硝酸盐的动物的血清葡萄糖和血糖糖化血红蛋白（HbA1c%）显著增加，而血清胰岛素和肝糖原减少，尤其是年轻组。还记录到体重增加和血清甲状腺激素（T4 和 T3）的显著减少。进一步显示血清尿素和肌酐以及血清、肝和胰腺中的总蛋白水平降低，同时所有暴露于硝酸盐的组的尿液中的这些物质水平升高。同时，血清转氨酶（ALT 和 AST）的活性增加，而肝组织中的其活性下降。此外，还记录到血清总胆红素、组织（肝和胰腺）一氧化氮和脂质谱以及葡萄糖-6-磷酸酶的肝活性升高。发现胡芦巴给药可有效缓解硝酸盐诱导的糖尿病的高血糖和其他生化变化。因此，胡芦巴可以被认为具有对抗硝酸盐诱导的糖尿病发生的有效作用。
