Ermolaev V I, Shumnyĭ T V, Miroshnichenko S M, Baranov O K
Genetika. 1988 Sep;24(9):1658-64.
Interspecific variability of the isotypic structure of two macroglobulins (Lpm and alpha 2M) in Mustelidae family was investigated using monospecific rabbit antisera against Lpm and alpha 2M Mustela vison. It was established that all the species studied may be divided into four groups, according to the degree of their homology with Lpm: 1) M. vison; 2) M. altaica, M. erminea, Kolonocus sibirica, Putorius eversmany, P. putorius, Luteola lutreola; 3) Vormela peregusna; 4) Martes martes, M. foina, M. zibellina. The greatest divergence of Martes gene species from M. vison and other species studied is determined by two different immunogenetic approaches, the first based on the whole isotype of Lpm and the other using allotypic markers of individual Lpm genes. The variability of the isotypic structure of alpha 2M was lower than that revealed in Lpm. V. peregusna is the only species which significantly differs from all above-mentioned species, including M. vison. The differences established for degree of variability between Lpm and alpha 2M in Mustelidae family may be connected with the evolutionary peculiarities of these two genetic system of serum alpha-macroglobulins, which being phylogenetically related, are still structurally divergent.
利用针对北美水貂Lpm和α2M的单特异性兔抗血清，研究了鼬科两种巨球蛋白（Lpm和α2M）同种型结构的种间变异性。结果表明，根据所研究物种与Lpm的同源程度，所有物种可分为四组：1）北美水貂；2）阿尔泰貂、白鼬、西伯利亚伶鼬、艾氏伶鼬、臭鼬、欧洲水獭；3）香鼬；4）松貂、黄喉貂、紫貂。通过两种不同的免疫遗传学方法确定了貂属基因物种与北美水貂及其他所研究物种之间的最大差异，第一种方法基于Lpm的全同种型，另一种方法使用单个Lpm基因的别型标记。α2M同种型结构的变异性低于Lpm中所揭示的变异性。香鼬是唯一与上述所有物种（包括北美水貂）显著不同的物种。鼬科中Lpm和α2M变异性程度的差异可能与血清α-巨球蛋白这两种遗传系统的进化特性有关，它们在系统发育上相关，但在结构上仍有差异。
