Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
1] Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA [2] Department of Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA.
Nat Mater. 2014 Apr;13(4):400-8. doi: 10.1038/nmat3890. Epub 2014 Mar 16.
The interface between plant organelles and non-biological nanostructures has the potential to impart organelles with new and enhanced functions. Here, we show that single-walled carbon nanotubes (SWNTs) passively transport and irreversibly localize within the lipid envelope of extracted plant chloroplasts, promote over three times higher photosynthetic activity than that of controls, and enhance maximum electron transport rates. The SWNT-chloroplast assemblies also enable higher rates of leaf electron transport in vivo through a mechanism consistent with augmented photoabsorption. Concentrations of reactive oxygen species inside extracted chloroplasts are significantly suppressed by delivering poly(acrylic acid)-nanoceria or SWNT-nanoceria complexes. Moreover, we show that SWNTs enable near-infrared fluorescence monitoring of nitric oxide both ex vivo and in vivo, thus demonstrating that a plant can be augmented to function as a photonic chemical sensor. Nanobionics engineering of plant function may contribute to the development of biomimetic materials for light-harvesting and biochemical detection with regenerative properties and enhanced efficiency.
细胞器与非生物纳米结构之间的界面有可能赋予细胞器新的增强功能。在这里，我们表明，单壁碳纳米管（SWNTs）被动地在提取的植物叶绿体的脂质包膜内运输并不可逆地定位，比对照物的光合作用活性提高了三倍以上，并增强了最大电子传递速率。SWNT-叶绿体组件还通过与增强的光吸收一致的机制，在体内实现更高的叶片电子传递速率。通过递送聚丙烯酸-纳米氧化铈或 SWNT-纳米氧化铈复合物，可显著抑制提取的叶绿体内部的活性氧浓度。此外，我们表明，SWNTs 可实现近红外荧光监测一氧化氮的体外和体内，从而证明植物可以被增强为光子化学传感器。植物功能的纳米仿生学工程可能有助于开发具有再生性能和增强效率的仿生材料，用于光捕获和生化检测。
