人角膜上皮细胞真菌感染过程中dectin-1的表达
Expression of dectin-1 during fungus infection in human corneal epithelial cells.
作者信息
Li Cui, Zhao Gui-Qiu, Che Cheng-Ye, Li Na, Lin Jing, Xu Qiang, Wang Qian, Liu Ying, Qiu Sheng
机构信息
Department of Ophthalmology, the Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China.
出版信息
Int J Ophthalmol. 2014 Feb 18;7(1):34-7. doi: 10.3980/j.issn.2222-3959.2014.01.06. eCollection 2014.
AIM
To evaluate the expression of dendritic cell-associated C-type lectin-1 (dectin-1) in human corneal epithelial (HCE) cells infected by fungus.
METHODS
A total of 20 cases of healthy donor corneas were group A, and 20 patients (20 eyes) suffered from fungal keratitis (FK) composed group B. Real-time qPCR and immunohistochemistry were applied to detect dectin-1 expression in corneal epithelium of both groups. HCE cells were cultured with aspergillus fumigatus (AF) antigens in vitro. The expression of dectin-1 mRNA was measured by real-time qPCR at the stimulation of 0, 4, 8 and 24h separately. Dectin-1 protein was detected by immunocytochemistry at 0 and 24h separately.
RESULTS
Dectin-1 expressed in corneal epithelium of normal persons and FK patients. Vitro cellular experiment showed that the expression of dectin-1 mRNA in HCE cells began to increase after stimulation of AF antigens at 4h, and dectin-1 protein expression increased after stimulation at 24h.
CONCLUSION
Dectin-1 expressed in corneal epithelium of normal persons. AF antigens stimulation can elevate the expression of dectin-1 in HCE cells in vitro.
目的
评估树突状细胞相关C型凝集素-1（dectin-1）在真菌感染的人角膜上皮（HCE）细胞中的表达。
方法
将20例健康供体角膜归为A组，20例真菌性角膜炎（FK）患者（20只眼）归为B组。应用实时定量聚合酶链反应（qPCR）和免疫组织化学检测两组角膜上皮中dectin-1的表达。体外将HCE细胞与烟曲霉（AF）抗原共培养。分别在刺激0、4、8和24小时时，通过实时qPCR检测dectin-1 mRNA的表达。分别在0和24小时时，通过免疫细胞化学检测dectin-1蛋白。
结果
dectin-1在正常人和FK患者的角膜上皮中均有表达。体外细胞实验表明，AF抗原刺激后4小时，HCE细胞中dectin-1 mRNA的表达开始增加，刺激24小时后dectin-1蛋白表达增加。
结论
dectin-1在正常人角膜上皮中表达。AF抗原刺激可在体外提高HCE细胞中dectin-1的表达。
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