Laporte Damien, Sagot Isabelle
Université de Bordeaux; Institut de Biochimie et Génétique Cellulaires; Bordeaux, France; CNRS; UMR5095 Bordeaux France; Bordeaux, France.
Nucleus. 2014 Mar-Apr;5(2):113-8. doi: 10.4161/nucl.28538. Epub 2014 Mar 14.
The nucleus is a cellular compartment that hosts several macro-molecular machines displaying a highly complex spatial organization. This tight architectural orchestration determines not only DNA replication and repair but also regulates gene expression. In budding yeast microtubules play a key role in structuring the nucleus since they condition the Rabl arrangement in G1 and chromosome partitioning during mitosis through their attachment to centromeres via the kinetochore proteins. Recently, we have shown that upon quiescence entry, intranuclear microtubules emanating from the spindle pole body elongate to form a highly stable bundle that spans the entire nucleus. Here, we examine some molecular mechanisms that may underlie the formation of this structure. As the intranuclear microtubule bundle causes a profound re-organization of the yeast nucleus and is required for cell survival during quiescence, we discuss the possibility that the assembly of such a structure participates in quiescence establishment.
细胞核是一个细胞区室，容纳着几种具有高度复杂空间组织的大分子机器。这种紧密的结构编排不仅决定了DNA复制和修复，还调节基因表达。在芽殖酵母中，微管在细胞核结构形成中起关键作用，因为它们通过动粒蛋白与着丝粒的附着，在G1期决定Rabl排列，并在有丝分裂期间调节染色体分配。最近，我们发现进入静止期后，从纺锤极体发出的核内微管会伸长，形成一个跨越整个细胞核的高度稳定的束状结构。在此，我们研究了这种结构形成可能的一些分子机制。由于核内微管束会引起酵母细胞核的深刻重组，并且是静止期细胞存活所必需的，我们讨论了这种结构的组装参与静止期建立的可能性。
