Navabazam Ali Reza, Sadeghian Nodoshan Fatemeh, Sheikhha Mohammad Hasan, Miresmaeili Sayyed Mohsen, Soleimani Mehrdad, Fesahat Farzaneh
Department of Oral and Maxillo-facial Surgery, Dental School, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Iran J Reprod Med. 2013 Mar;11(3):235-42.
Human dental stem cells have high proliferative potential for self-renewal that is important to the regenerative capacity of the tissue. Objective : The aim was to isolate human dental pulp stem cells (DPSC), periodontal ligament stem cells (PDLSC) and periapical follicle stem cells (PAFSC) for their potential role in tissue regeneration.
METHODS & MATERIALS: In this experimental study, the postnatal stem cells were isolated from dental pulp, preapical follicle and periodontal ligament .The cells were stained for different stem cell markers by immunocytochemistry. To investigate the mesenchymal nature of cells, differentiation potential along osteoblastic and adipogenic lineages and gene expression profile were performed. For proliferation potential assay, Brdu staining and growth curve tests were performed. Finally, all three cell types were compared together regarding their proliferation, differentiation and displaying phenotype.
The isolated cell populations have similar fibroblastic like morphology and expressed all examined cell surface molecule markers. These cells were capable of differentiating into osteocyte with different capability and adipocyte with the same rate. PAFSCs showed more significant proliferation rate than others. Reverse transcriptase PCR (RT-PCR) for nanog, oct4, Alkaline phosphatase (ALP) and glyceraldehydes-3-phosphate dehydrogenease (GADPH) as control gene showed strong positive expression of these genes in all three isolated cell types.
PDLSCs, DPSCs and PAFSCs exist in various tissues of the teeth and can use as a source of mesenchymal stem cells for developing bioengineered organs and also in craniomaxillofacial reconstruction with varying efficiency in differentiation and proliferation.
人类牙髓干细胞具有高度增殖的自我更新潜能，这对组织的再生能力至关重要。目的：旨在分离人牙髓干细胞（DPSC）、牙周膜干细胞（PDLSC）和根尖周滤泡干细胞（PAFSC），以研究它们在组织再生中的潜在作用。
在本实验研究中，从牙髓、根尖周滤泡和牙周膜中分离出生后干细胞。通过免疫细胞化学对细胞进行不同干细胞标志物染色。为研究细胞的间充质性质，进行了沿成骨和成脂谱系的分化潜能及基因表达谱分析。对于增殖潜能分析，进行了溴脱氧尿苷（Brdu）染色和生长曲线测试。最后，比较了这三种细胞类型在增殖、分化和表型方面的差异。
分离出的细胞群体具有相似的成纤维细胞样形态，并表达所有检测的细胞表面分子标志物。这些细胞能够以不同能力分化为骨细胞，并以相同速率分化为脂肪细胞。PAFSCs显示出比其他细胞更高的增殖率。以纳米管蛋白（nanog）、八聚体结合转录因子4（oct4）、碱性磷酸酶（ALP）和甘油醛-3-磷酸脱氢酶（GADPH）作为对照基因的逆转录聚合酶链反应（RT-PCR）显示，这三种分离的细胞类型中这些基因均呈强阳性表达。
PDLSCs、DPSCs和PAFSCs存在于牙齿的不同组织中，可作为间充质干细胞来源用于开发生物工程器官，也可用于颅颌面重建，但其在分化和增殖方面的效率各不相同。
