De Assis-Paiva H J, Champion B, Rayner D C, Colle E, Bone A, Roitt I M, Cooke A
Department of Immunology, University College and Middlesex School of Medicine, London.
Clin Exp Immunol. 1988 Oct;74(1):63-7.
We have investigated the epitope specificities of rat thyroglobulin (Tg) autoantibodies arising either spontaneously in BB hybrid and BB rats or following induction in normal rats with thyroglobulin and adjuvant. Using a panel of thyroglobulins from different animal species it was possible to identify three different patterns of reactivity. These were: 1) recognition of all species of thyroglobulin; (2) recognition restricted to rat and mouse thyroglobulins and 3) recognition biased towards dog, rat and mouse thyroglobulins. Furthermore, using human thyroglobulin manifesting different levels of iodination, it was possible to show that sera with recognition pattern 1 recognized the iodination site of thyroglobulin and that this was inhibitable by thyroxine. Taken together these data provide evidence of restricted epitope recognition by Tg autoantibodies in the rat.
我们研究了BB杂种大鼠和BB大鼠自发产生的，或正常大鼠用甲状腺球蛋白和佐剂诱导产生的大鼠甲状腺球蛋白（Tg）自身抗体的表位特异性。使用一组来自不同动物物种的甲状腺球蛋白，有可能识别出三种不同的反应模式。它们分别是：1）识别所有物种的甲状腺球蛋白；（2）识别仅限于大鼠和小鼠的甲状腺球蛋白；3）识别偏向于狗、大鼠和小鼠的甲状腺球蛋白。此外，使用表现出不同碘化水平的人甲状腺球蛋白，有可能表明具有反应模式1的血清识别甲状腺球蛋白的碘化位点，并且这可被甲状腺素抑制。这些数据综合起来提供了大鼠中Tg自身抗体对表位识别受限的证据。
