Li Yanjie, Li Pan, Wang Yong, Dong Ruirui, Yu Huimin, Hou Bingkai
The Key Lab of Plant Cell Engineering and Germplasm Innovation, Education Ministry of China, Jinan, 250100, Shandong, People's Republic of China,
Planta. 2014 Jun;239(6):1265-79. doi: 10.1007/s00425-014-2050-1. Epub 2014 Mar 20.
Family-1 UDP glycosyltransferases (UGTs) from plants transfer sugar moieties from activated sugar donors to a wide range of small molecules, and control many metabolic processes during plant growth and development. Here, we report a genome-wide analysis of maize that identified 147 Family-1 glycosyltransferases based on their conserved PSPG motifs. Phylogenetic analysis of these genes with 18 Arabidopsis UGTs and two rice UGTs clustered them into 17 groups (A-Q). The patterns of intron gain/loss events, as well as their positions within UGTs from the same group, further aided elucidation of their divergence and evolutionary relationships between UGTs. Expression analysis of the maize UGT genes using both online microarray data and quantitative real-time PCR verification indicates that UGT genes are widely expressed in various tissues and likely play important roles in plant growth and development. Our study provides useful information on the Family-1 UGTs in maize, and will facilitate their further characterization to better understand their functions.
植物中的1类UDP糖基转移酶（UGTs）将活化糖供体中的糖基部分转移到多种小分子上，并控制植物生长发育过程中的许多代谢过程。在此，我们报告了一项对玉米的全基因组分析，该分析基于其保守的PSPG基序鉴定出147个1类糖基转移酶。对这些基因与18个拟南芥UGTs和2个水稻UGTs进行系统发育分析，将它们聚类为17个组（A - Q）。内含子获得/丢失事件的模式以及它们在同一组UGTs中的位置，进一步有助于阐明UGTs之间的差异和进化关系。使用在线微阵列数据和定量实时PCR验证对玉米UGT基因进行的表达分析表明，UGT基因在各种组织中广泛表达，并且可能在植物生长发育中发挥重要作用。我们的研究为玉米中的1类UGTs提供了有用信息，并将有助于对它们进行进一步表征，以更好地了解其功能。
