Emanuelli G, Montrucchio G, Gaia E, Dughera L, Corvetti G, Gubetta L
Clinica Medica Generale, Universita' di Torino, Ospedale S. Luigi Gonzaga, Orbassano, Italy.
Am J Pathol. 1989 Feb;134(2):315-26.
This study indicates that a single injection of platelet activating factor (PAF, 50-500 ng) into the superior pancreaticoduodenal artery of rabbits induces dose-dependent morphologic alterations of pancreatic tissue and increases serum amylase levels, both consistent with the development of an acute pancreatitis. The main histologic findings observed by light microscopy 24-72 hours after the injection of PAF were edema, polymorphonuclear neutrophil infiltration, cell vacuolization, and acinar cell necrosis. Fat cell necrosis was present in 30% of animals. By electron microscopy an increase of the number of zymogen granules in the apical region of acinar cells was observed 3 hours after PAF challenge. At 24-72 hours, many acinar cells showed vacuoles containing myelinlike figures, zymogen granules, and cellular debris. Pancreatic lesions developed in the area supplied by the artery injected with PAF and they were completely antagonized by the pretreatment of rabbits with CV 3988, a specific antagonist of PAF. In addition, the significant protective effect of atropine suggests a potential role for cholinergic mechanisms in the pancreatic alterations induced by PAF.
本研究表明，向兔胰十二指肠上动脉单次注射血小板活化因子（PAF，50 - 500 ng）可诱导胰腺组织出现剂量依赖性形态学改变，并使血清淀粉酶水平升高，两者均与急性胰腺炎的发展一致。注射PAF后24 - 72小时，光镜下观察到的主要组织学表现为水肿、多形核中性粒细胞浸润、细胞空泡化和腺泡细胞坏死。30%的动物出现脂肪细胞坏死。电镜观察发现，PAF刺激3小时后，腺泡细胞顶端区域的酶原颗粒数量增加。在24 - 72小时时，许多腺泡细胞出现含有髓鞘样结构、酶原颗粒和细胞碎片的空泡。胰腺病变发生在注射PAF的动脉所供应的区域，并且用PAF的特异性拮抗剂CV 3988对兔进行预处理可完全拮抗这些病变。此外，阿托品的显著保护作用提示胆碱能机制在PAF诱导的胰腺改变中可能发挥作用。
