Shi Shuobo, Zhao Huimin
Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing, China.
Metabolic Engineering Research Laboratory, Science and Engineering Institutes, Agency for Science, Technology and Research, Singapore, Singapore.
Front Microbiol. 2017 Nov 8;8:2185. doi: 10.3389/fmicb.2017.02185. eCollection 2017.
Oleaginous yeasts have been increasingly explored for production of chemicals and fuels via metabolic engineering. Particularly, there is a growing interest in using oleaginous yeasts for the synthesis of lipid-related products due to their high lipogenesis capability, robustness, and ability to utilize a variety of substrates. Most of the metabolic engineering studies in oleaginous yeasts focused on that already has plenty of genetic engineering tools. However, recent advances in systems biology and synthetic biology have provided new strategies and tools to engineer those oleaginous yeasts that have naturally high lipid accumulation but lack genetic tools, such as , , and . This review highlights recent accomplishments in metabolic engineering of oleaginous yeasts and recent advances in the development of genetic engineering tools in oleaginous yeasts within the last 3 years.
产油酵母已越来越多地被用于通过代谢工程生产化学品和燃料。特别是，由于其高脂质生成能力、耐受性以及利用多种底物的能力，利用产油酵母合成脂质相关产品的兴趣日益浓厚。大多数关于产油酵母的代谢工程研究都集中在那些已经有大量基因工程工具的酵母上。然而，系统生物学和合成生物学的最新进展提供了新的策略和工具，用于对那些天然脂质积累高但缺乏基因工具的产油酵母进行工程改造，如[具体酵母名称1]、[具体酵母名称2]和[具体酵母名称3]。本综述重点介绍了产油酵母代谢工程的最新成果以及过去3年中产油酵母基因工程工具开发的最新进展。
