Kuga T, Yamasaki M, Sekine S, Fukui M, Yokoo Y, Itoh S, Yoshida M, Hattori T, Takatsuki K
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Tokyo.
Jpn J Cancer Res. 1988 Nov;79(11):1168-73. doi: 10.1111/j.1349-7006.1988.tb01541.x.
A fused gene of the gag and env sequences of human T-cell leukemia virus type I (HTLV-I), the causative agent of adult T-cell leukemia, was constructed in vitro and expressed in Escherichia coli. The gag-env hybrid protein accumulated as insoluble granules with a yield of approximately 12% of the total proteins. In an enzyme-linked immunosorbent assay done with the use of the gag-env hybrid protein, all 57 seropositive sera gave positive signals, and none of the sera from normal persons did. This system can produce large quantities of the gag-env hybrid protein, which can be used for mass screening of human sera for HTLV-I infection.
成人T细胞白血病的病原体——人类T细胞白血病病毒I型（HTLV-I）的gag和env序列的融合基因在体外构建并在大肠杆菌中表达。gag-env杂交蛋白以不溶性颗粒形式积累，产量约占总蛋白的12%。在用gag-env杂交蛋白进行的酶联免疫吸附试验中，所有57份血清反应阳性的血清均给出阳性信号，而正常人的血清均未给出阳性信号。该系统可大量生产gag-env杂交蛋白，可用于大规模筛查人血清中的HTLV-I感染。
