Nikolaeva-Glomb Lubomira, Mukova Luchia, Nikolova Nadya, Badjakov Ilian, Dincheva Ivayla, Kondakova Violeta, Doumanova Lyuba, Galabov Angel S
Nat Prod Commun. 2014 Jan;9(1):51-4.
Wild berry species are known to exhibit a wide range of pharmacological activities. They have long been traditionally applied for their antiseptic, antimicrobial, cardioprotective and antioxidant properties. The aim of the present study is to reveal the potential for selective antiviral activity of total methanol extracts, as well as that of the anthocyanins and the non-anthocyanins from the following wild berries picked in Bulgaria: strawberry (Fragaria vesca L.) and raspberry (Rubus idaeus L.) of the Rosaceae plant family, and bilberry (Vaccinium myrtillis L.) and lingonberry (Vaccinium vitis-idaea L) of the Ericaceae. The antiviral effect has been tested against viruses that are important human pathogens and for which chemotherapy and/or chemoprophylaxis is indicated, namely poliovirus type 1 (PV-1) and coxsackievirus B1 (CV-B1) from the Picornaviridae virus family, human respiratory syncytial virus A2 (HRSV-A2) from the Paramyxoviridae and influenza virus A/H3N2 of Orthomyxoviridae. Wild berry fruits are freeze-dried and ground, then total methanol extracts are prepared. Further the extracts are fractioned by solid phase extraction and the non-anthocyanin and anthocyanin fractions are eluted. The in vitro antiviral effect is examined by the virus cytopathic effect (CPE) inhibition test. The results reveal that the total extracts of all tested berry fruits inhibit the replication of CV-B1 and influenza A virus. CV-B1 is inhibited to the highest degree by both bilberry and strawberry, as well as by lingonberry total extracts, and influenza A by bilberry and strawberry extracts. Anthocyanin fractions of all wild berries strongly inhibit the replication of influenza virus A/H3N2. Given the obtained results it is concluded that wild berry species are a valuable resource of antiviral substances and the present study should serve as a basis for further detailed research on the matter.
已知野生浆果品种具有广泛的药理活性。长期以来，它们一直因其防腐、抗菌、心脏保护和抗氧化特性而被传统应用。本研究的目的是揭示从保加利亚采摘的以下野生浆果中总甲醇提取物以及花青素和非花青素的选择性抗病毒活性潜力：蔷薇科植物家族的草莓（Fragaria vesca L.）和覆盆子（Rubus idaeus L.），以及杜鹃花科的越橘（Vaccinium myrtillis L.）和欧洲越橘（Vaccinium vitis-idaea L）。已经针对作为重要人类病原体且需要化疗和/或化学预防的病毒测试了抗病毒效果，即小核糖核酸病毒科的1型脊髓灰质炎病毒（PV-1）和柯萨奇病毒B1（CV-B1）、副粘病毒科的人呼吸道合胞病毒A2（HRSV-A2）以及正粘病毒科的甲型流感病毒A/H3N2。将野生浆果果实冷冻干燥并研磨，然后制备总甲醇提取物。进一步通过固相萃取对提取物进行分离，并洗脱非花青素和花青素部分。通过病毒细胞病变效应（CPE）抑制试验检查体外抗病毒效果。结果表明，所有测试浆果果实的总提取物均抑制CV-B1和甲型流感病毒的复制。越橘和草莓以及欧洲越橘的总提取物对CV-B1的抑制程度最高，越橘和草莓提取物对甲型流感病毒的抑制作用最强。所有野生浆果的花青素部分强烈抑制甲型流感病毒A/H3N2的复制。鉴于所获得的结果，可以得出结论，野生浆果品种是抗病毒物质的宝贵资源，本研究应为该问题的进一步详细研究奠定基础。
