Satta G, Pompei R, Grazi G, Cornaglia G
Istituto di Microbiologia, Università degli Studi di Siena, Italy.
J Clin Microbiol. 1988 Dec;26(12):2637-41. doi: 10.1128/jcm.26.12.2637-2641.1988.
In this study we evaluated phosphatase activity in members of the family Enterobacteriaceae by conventional methods and by a novel method. The novel method is based on the formation of bright-green-strained colonies by phosphatase-positive, but not phosphatase-negative, strains in the presence of a phosphate substrate, such as phenolphthalein monophosphate or 6-benzoylnaphthyl phosphate (6-BNP), and methyl green. A total of 1,055 strains belonging to 65 different species of Enterobacteriaceae were tested for green staining of the colonies in the presence of methyl green and either phenolphthalein monophosphate or 6-BNP and for phosphatase activity by three different conventional methods. With the sole exception of one Leminorella richardii type strain, all isolates of all of the species formed green-stained colonies in the presence of the substrate 6-BNP. All strains were phosphatase positive by all of the conventional methods.
在本研究中，我们通过传统方法和一种新方法评估了肠杆菌科成员的磷酸酶活性。新方法基于在存在磷酸底物（如酚酞单磷酸酯或6-苯甲酰萘基磷酸酯（6-BNP））和甲基绿的情况下，磷酸酶阳性菌株而非磷酸酶阴性菌株形成亮绿色染色菌落。对总共属于65种不同肠杆菌科物种的1055株菌株进行了检测，检测其在存在甲基绿和酚酞单磷酸酯或6-BNP的情况下菌落的绿色染色情况，以及通过三种不同的传统方法检测磷酸酶活性。除了一株理查德利米诺菌模式菌株外，所有物种的所有分离株在存在底物6-BNP的情况下均形成绿色染色菌落。通过所有传统方法检测，所有菌株的磷酸酶均呈阳性。
