Leoni Livia, Landini Paolo
Department of Sciences, Università "Roma Tre", Rome, Italy.
Methods Mol Biol. 2014;1147:175-86. doi: 10.1007/978-1-4939-0467-9_12.
The ability of many pathogenic bacteria to grow as a biofilm results in lower susceptibility to antibiotic treatments and to the host immune response, thus leading to the development of chronic infections. The understanding that biofilms can play an important role in bacterial virulence has prompted the search for inhibitors of biofilm development and of biofilm-related cellular processes. In this report, we present two examples of target-based microbiological screenings for antimicrobials endowed with anti-biofilm activity, aimed respectively at the inhibition of the signal molecule cyclic di-GMP and of quorum sensing.
许多致病细菌形成生物膜生长的能力导致其对抗生素治疗和宿主免疫反应的敏感性降低，从而引发慢性感染。认识到生物膜在细菌致病性中可发挥重要作用，促使人们寻找生物膜形成及与生物膜相关细胞过程的抑制剂。在本报告中，我们展示了两种基于靶点的微生物筛选方法，用于筛选具有抗生物膜活性的抗菌剂，分别针对信号分子环二鸟苷酸的抑制和群体感应的抑制。
