使用颜色校准玻片进行全玻片成像中的颜色标准化。
Color standardization in whole slide imaging using a color calibration slide.
作者信息
Bautista Pinky A, Hashimoto Noriaki, Yagi Yukako
机构信息
Department of Pathology, Massachusetts General Hospital, Boston, MA 02114, USA.
出版信息
J Pathol Inform. 2014 Jan 31;5(1):4. doi: 10.4103/2153-3539.126153. eCollection 2014.
BACKGROUND
Color consistency in histology images is still an issue in digital pathology. Different imaging systems reproduced the colors of a histological slide differently.
MATERIALS AND METHODS
Color correction was implemented using the color information of the nine color patches of a color calibration slide. The inherent spectral colors of these patches along with their scanned colors were used to derive a color correction matrix whose coefficients were used to convert the pixels' colors to their target colors.
RESULTS
There was a significant reduction in the CIELAB color difference, between images of the same H & E histological slide produced by two different whole slide scanners by 3.42 units, P < 0.001 at 95% confidence level.
CONCLUSION
Color variations in histological images brought about by whole slide scanning can be effectively normalized with the use of the color calibration slide.
背景
在数字病理学中，组织学图像的颜色一致性仍然是一个问题。不同的成像系统对组织学切片颜色的再现方式不同。
材料与方法
利用色彩校准玻片上九个色块的颜色信息进行色彩校正。这些色块的固有光谱颜色及其扫描颜色被用于推导一个色彩校正矩阵，其系数用于将像素颜色转换为目标颜色。
结果
在95%置信水平下，由两台不同的全切片扫描仪生成的同一张苏木精和伊红（H&E）组织学切片图像之间，CIELAB色差显著降低了3.42个单位，P < 0.001。
结论
使用色彩校准玻片可以有效地归一化全切片扫描带来的组织学图像颜色变化。
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