Haselman T, Camp D G, Fox G E
Department of Biochemical and Biophysical Sciences, University of Houston, TX 77204-5500.
Nucleic Acids Res. 1989 Mar 25;17(6):2215-21. doi: 10.1093/nar/17.6.2215.
Major efforts are underway to elucidate the spatial distribution of ribosomal RNAs (rRNAs) in the ribosome. An especially informative approach is the identification of likely base-base tertiary interactions within the RNA by phylogenetic comparison. Herein evidence is presented for three heretofore unrecognized candidate tertiary interactions, G506/C525, C779/G803 and A994/U1380 (1) in 16S-like rRNAs. This brings to eight the number of such interactions that are strongly supported by phylogenetic evidence. The three newly identified interactions further define the folding within domains II and III of 16S-like rRNA. No interactions have yet been found that would serve to orient the domains relative to one another.
目前正在做出重大努力来阐明核糖体中核糖体RNA(rRNA)的空间分布。一种特别有用的方法是通过系统发育比较来识别RNA内可能的碱基-碱基三级相互作用。本文提供了证据,证明在16S样rRNA中存在三种迄今未被识别的候选三级相互作用,即G506/C525、C779/G803和A994/U1380(1)。这使得系统发育证据强烈支持的此类相互作用的数量增加到了八个。新发现的这三种相互作用进一步确定了16S样rRNA结构域II和III内的折叠情况。尚未发现有相互作用可用于使这些结构域相互定向。