Spadiut Oliver, Herwig Christoph
Vienna University of Technology, Institute of Chemical Engineering, Research Area Biochemical Engineering, Gumpendorfer Strasse 1a, A-1060 Vienna, Austria.
Pharm Bioprocess. 2013 Aug 1;1(3):283-295. doi: 10.4155/pbp.13.23.
The oxidoreductase horseradish peroxidase (HRP) is used in numerous industrial and medical applications. In this review, we briefly describe this well-studied enzyme and focus on its promising use in targeted cancer treatment. In combination with a plant hormone, HRP can be used in specific enzyme-prodrug therapies. Despite this outstanding application, HRP has not found its way as a biopharmaceutical into targeted cancer therapy yet. The reasons therefore lie in the present low-yield production and cumbersome purification of this enzyme from its natural source. However, surface glycosylation renders the recombinant production of HRP difficult. Here, we compare different production hosts for HRP and summarize currently used production and purification strategies for this enzyme. We further present our own strategy of glycoengineering this powerful enzyme to allow recombinant high-yield production in and subsequent simple downstream processing.
氧化还原酶辣根过氧化物酶（HRP）在众多工业和医学应用中都有使用。在本综述中，我们简要描述这种经过充分研究的酶，并重点关注其在靶向癌症治疗中的潜在用途。与植物激素结合，HRP可用于特定的酶-前药疗法。尽管有这种出色的应用，但HRP尚未作为生物药物进入靶向癌症治疗领域。其原因在于目前从天然来源生产该酶的产量低且纯化繁琐。然而，表面糖基化使得HRP的重组生产变得困难。在此，我们比较了HRP的不同生产宿主，并总结了目前用于该酶的生产和纯化策略。我们还展示了我们自己对这种强大酶进行糖基工程的策略，以实现其在[具体宿主]中的重组高产生产以及随后简单的下游加工。
