Departamento de Genética, Universidade Federal do Paraná, Curitiba, PR, Brazil . ; Escola de Saúde e Biociências, Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil .
Escola de Saúde e Biociências, Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil . ; Departamento de Educação Física, Universidade Federal do Paraná, Curitiba, PR, Brazil .
Genet Mol Biol. 2014 Mar;37(1):15-22. doi: 10.1590/s1415-47572014000100005. Epub 2013 Feb 28.
The individual response to diet may be influenced by gene polymorphisms. This study hypothesized that ADRB2 (Gln27Glu, rs1042714 and Arg16Gly, rs1042713), ADRB3 (Trp64Arg, rs4994) and GHRL (Leu72Met, rs696217) polymorphisms moderate weight loss. The study was a seven weeks dietary weight loss intervention with Brazilian adult obese women (n = 109). The body mass index (BMI) was calculated and polymorphisms in these genes were assessed by real-time PCR assays. Two-way repeated-measures ANOVA (2 × 2) were used to analyze the intervention effect between polymorphisms and BMI over the period and after stratification for age and socioeconomic status (SES). The weight loss intervention resulted in decreased BMI over the seven-week period (p < 0.001), for high and low SES (p < 0.05) and mainly for participants with 30-49 y. The intervention did not result in a statistically significant difference in weight loss between polymorphism carriers and non-carriers, and although, the ADRB2, ADRB3 and GHRL polymorphisms did not moderate weight loss, the Gln27Glu polymorphism carriers showed a lower BMI compared to non-carriers in the low SES (p = 0.018) and the 30-39 y (p = 0.036) groups, suggesting a role for this polymorphism related to BMI control.
个体对饮食的反应可能受到基因多态性的影响。本研究假设 ADRB2（Gln27Glu、rs1042714 和 Arg16Gly、rs1042713）、ADRB3（Trp64Arg、rs4994）和 GHRL（Leu72Met、rs696217）多态性可以调节体重减轻。该研究是一项为期七周的饮食减肥干预研究，对象为巴西成年肥胖女性（n=109）。通过实时 PCR 检测这些基因的多态性，计算体重指数（BMI）。采用双因素重复测量方差分析（2×2）分析了该多态性与 BMI 之间的干预效果，研究时间跨度为 7 周，并按年龄和社会经济地位（SES）进行了分层。体重减轻干预在七周内导致 BMI 下降（p<0.001），高 SES 和低 SES 组均如此（p<0.05），主要影响 30-49 岁的参与者。干预并没有导致多态性携带者和非携带者之间的体重减轻存在统计学差异，尽管 ADRB2、ADRB3 和 GHRL 多态性不能调节体重减轻，但低 SES（p=0.018）和 30-39 岁（p=0.036）组的 Gln27Glu 多态性携带者的 BMI 低于非携带者，这表明该多态性与 BMI 控制有关。
