使用非共焦自适应光学扫描激光检眼镜对视网膜血管结构和灌注进行可视化。
Visualization of retinal vascular structure and perfusion with a nonconfocal adaptive optics scanning light ophthalmoscope.
作者信息
Sulai Yusufu N, Scoles Drew, Harvey Zachary, Dubra Alfredo
出版信息
J Opt Soc Am A Opt Image Sci Vis. 2014 Mar 1;31(3):569-79. doi: 10.1364/JOSAA.31.000569.
Abstract
Imaging of the retinal vascular structure and perfusion was explored by confocal illumination and nonconfocal detection in an adaptive optics scanning light ophthalmoscope (AOSLO), as an extension of the work by Chui et al. [Biomed. Opt. Express 3, 2537 (2012)]. Five different detection schemes were evaluated at multiple retinal locations: circular mask, annular mask, circular mask with filament, knife-edge, and split-detector. Given the superior image contrast in the reflectance and perfusion maps, the split-detection method was further tested using pupil apodization, polarized detection, and four different wavelengths. None of these variations provided noticeable contrast improvement. The noninvasive visualization of capillary flow and structure provided by AOSLO split-detection shows great promise for studying ocular and systemic conditions that affect the retinal vasculature.
摘要
作为Chui等人[《生物医学光学快报》3, 2537 (2012)]工作的扩展，在自适应光学扫描激光检眼镜(AOSLO)中通过共焦照明和非共焦检测来探索视网膜血管结构和灌注的成像。在多个视网膜位置评估了五种不同的检测方案：圆形掩膜、环形掩膜、带细丝的圆形掩膜、刀口和分裂探测器。鉴于反射率和灌注图中具有优异的图像对比度，使用瞳孔切趾术、偏振检测和四种不同波长对分裂检测方法进行了进一步测试。这些变化均未带来显著的对比度改善。AOSLO分裂检测提供的毛细血管血流和结构的无创可视化对于研究影响视网膜血管系统的眼部和全身状况具有很大的前景。
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