Research Organization for Infection Control Sciences, Kitasato University, Tokyo, Japan.
Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan.
J Antibiot (Tokyo). 2014 Jul;67(7):533-9. doi: 10.1038/ja.2014.34. Epub 2014 Apr 2.
We have been continually searching for novel chemical compounds from culture broths of various actinomycetes using a physicochemical screening system. During the course of this program, we have previously reported the discovery of two new natural products, designated mangromicins A and B, discovered in a broth of a rare actinomycete strain, Lechevalieria aerocolonigenes K10-0216. Mangromicins have a unique and rare structure, a cyclopentadecane skeleton with a tetrahydrofuran unit and a 5,6-dihydro-4-hydroxy-2-pyrone moiety. New mangromicin analogs were isolated by using an improved production medium. As a consequence, six analogs, together with mangromicins A and B, were isolated from a cultured broth of L. aerocolonigenes K10-0216. We named them mangromicins D, E, F, G, H and I. All mangromicins showed radical scavenging activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals and nitric oxide generated from LPS-stimulated RAW264.7 cells, a murine macrophage cell line. Among the analogs, mangromicins A and I showed the most potent DPPH radical scavenging activity and nitric oxide scavenging activity, respectively.
我们一直在使用理化筛选系统从各种放线菌的发酵液中不断寻找新的化合物。在这个项目的过程中，我们之前曾报道过两种新的天然产物的发现，它们被命名为 mangromicins A 和 B，是从一种稀有放线菌菌株 Lechevalieria aerocolonigenes K10-0216 的发酵液中发现的。Mangromicins 具有独特而罕见的结构，一个环十五烷骨架，带有一个四氢呋喃单元和一个 5,6-二氢-4-羟基-2-吡喃酮部分。通过使用改良的生产培养基分离到了新的 mangromicin 类似物。结果，从 L. aerocolonigenes K10-0216 的培养肉汤中分离出了六种类似物，以及 mangromicins A 和 B。我们将它们命名为 mangromicins D、E、F、G、H 和 I。所有的 mangromicins 都显示出对 1,1-二苯基-2-苦基肼（DPPH）自由基和 LPS 刺激的 RAW264.7 细胞（一种鼠巨噬细胞系）产生的一氧化氮的清除自由基活性。在这些类似物中，mangromicins A 和 I 分别显示出最强的 DPPH 自由基清除活性和一氧化氮清除活性。
