Department of Stomatology, The 940th Hospital of Joint Logistic Support Force of the Chinese People's Liberation Army, Lanzhou, 730050, PR China.
School of Stomatology, Lanzhou University, Lanzhou, 730000, PR China.
Dent Mater. 2021 Apr;37(4):636-647. doi: 10.1016/j.dental.2021.01.007. Epub 2021 Feb 10.
The core-shell chlorhexidine/amorphous calcium phosphate (CHX/ACP) nanoparticles were synthesized and used to modify the dental resin composite, aiming to improve its remineralized and antibacterial properties.
The core-shell CHX/ACP nanoparticles were synthesized by vesicle-templating technology and characterized, and their sustained release and antibacterial properties were also evaluated. Subsequently, the synthesized nanoparticles were incorporated into the dental resin composite at 1 wt.%, 5 wt.% or 10 wt.% to obtain different experimental groups. The physical properties, including curing depth, double bond conversion rate, water absorption and solubility, the sustained-release effects, and mechanical properties of the modified resin composite were evaluated. The remineralization ability was also measured by SEM. The antibacterial experiment of the modified resin composite with fresh preparation or aging in water for 28 days was carried out by a plate count method.
The physical and chemical characterizations showed that the synthesized nanoparticles presented a core-shell structure, and their diameter was about 98.5 nm. The shell was composed of ACP with the core full of CHX. These nanoparticles had a release effect on calcium, phosphate ions, and CHX. The nanoparticles could effectively inhibit the growth of S. mutan at a lower concentration (≥50 μg/mL). The curing depth, the double bond conversion, the water absorption, the solubility, the flexural strength, the flexural modulus, and the compressive strength of the modified resin composite were 3.86-4.88 mm, 62.32-73.61%, 1.47-2.84%, 0.21-0.48%, 45.83-109.46 MPa, 2.57-4.91 GPa, and 66.43-160.38 MPa, respectively. The modified resin composite containing 5 wt.% and above CHX/ACP nanoparticles could effectively inhibit the growth of S. mutans regardless of aging in water, with immediate and aging antibacterial rate of more than 92%. In addition, the modified resin composite had a certain remineralization property in the SBF solution verified by SEM.
The core-shell CHX/ACP nanoparticles were successfully prepared and used to modify the resin composite. The modified dental resin composite with 5 wt.% CHX/ACP nanoparticles had excellent mechanical, antibacterial, and remineralization properties. It is expected to be an ideal restorative filling material for clinical application.
合成核壳型氯己定/无定形磷酸钙（CHX/ACP）纳米粒子，并将其用于修饰牙用树脂复合材料，以提高其再矿化和抗菌性能。
采用囊泡模板技术合成核壳型 CHX/ACP 纳米粒子，并对其进行表征，同时评价其缓释性能和抗菌性能。然后，将合成的纳米粒子以 1wt.%、5wt.%或 10wt.%的比例掺入牙用树脂复合材料中，得到不同实验组。评估改性树脂复合材料的物理性能，包括固化深度、双键转化率、吸水率和溶解度、缓释效果以及机械性能。通过 SEM 测量其再矿化能力。通过平板计数法对新鲜制备或在水中老化 28 天的改性树脂复合材料进行抗菌实验。
理化特性分析表明，所合成的纳米粒子呈核壳结构，直径约为 98.5nm。壳层由 ACP 组成，核心充满 CHX。这些纳米粒子对钙、磷酸盐离子和 CHX 具有释放作用。纳米粒子在较低浓度（≥50μg/ml）下即可有效抑制变形链球菌的生长。改性树脂复合材料的固化深度、双键转化率、吸水率、溶解度、弯曲强度、弯曲模量和抗压强度分别为 3.86-4.88mm、62.32-73.61%、1.47-2.84%、0.21-0.48%、45.83-109.46MPa、2.57-4.91GPa 和 66.43-160.38MPa。当改性树脂复合材料中 CHX/ACP 纳米粒子的含量达到 5wt.%及以上时，无论是否在水中老化，均能有效抑制变形链球菌的生长，即时和老化后的抑菌率均超过 92%。此外，通过 SEM 验证，改性树脂复合材料在 SBF 溶液中具有一定的再矿化性能。
成功制备了核壳型 CHX/ACP 纳米粒子，并将其用于修饰树脂复合材料。当改性树脂复合材料中 CHX/ACP 纳米粒子的含量为 5wt.%时，其具有优异的机械性能、抗菌性能和再矿化性能，有望成为一种理想的临床应用修复填充材料。
