Wong-Villarreal Arnoldo, Méndez-Santiago Erick Williams, Gómez-Rodríguez Olga, Aguilar-Marcelino Liliana, García Daniel Cerqueda, García-Maldonado José Q, Hernández-Velázquez Victor M, Yañez-Ocampo Gustavo, Espinosa-Zaragoza Saúl, I Ramírez-González Sandra, Sanzón-Gómez Diana
División Agroalimentaria, Universidad Tecnológica de la Selva, Ocosingo 29950, Mexico.
Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca 62209, Mexico.
Plants (Basel). 2021 Dec 3;10(12):2655. doi: 10.3390/plants10122655.
The genus is widely distributed in soil, water, plants, animals, invertebrates, and humans. Some species of this genus have antifungal, antibacterial, and nematicidal activity. In this work, the nematicidal activity of the endophytic strain of sp. in chili, L., is reported, where at a bacterial concentration of 4 × 10 cel/mL, the penetration of nematodes into the roots significantly decreased by 91 and 55% at 7 and 21 days after inoculation. This bacterial concentration also significantly decreased the number of galls, eggs, egg masses and reproduction factor produced by in Chili plants, with respect to the control where this bacterial strain was not applied. In the analysis of the genome of the strain, based on average nucleotide identity (ANI), the isolate could be affiliated to the species . The size of the genome is 5.4 Mb, with a 59.3% content of GC. Genes related to the synthesis of chitinases, siderophores, proteases C, serralisins, hemolysin, and serrawettin W2 that have been reported for biocontrol of nematodes were identified in the genome. It is the first report of with nematicidal activity. Based on these results of nematicidal activity, this strain can be evaluated in the field as an alternative in the biocontrol of in chili cultivation.
该属广泛分布于土壤、水、植物、动物、无脊椎动物和人类中。该属的一些物种具有抗真菌、抗菌和杀线虫活性。在本研究中，报道了辣椒内生菌株sp.对辣椒（L.）的杀线虫活性，接种后7天和21天，当细菌浓度为4×10细胞/毫升时，线虫对根的侵入显著降低了91%和55%。与未施用该菌株的对照相比，该细菌浓度还显著降低了辣椒植株中由引起的虫瘿、卵、卵块数量和繁殖系数。在菌株基因组分析中，基于平均核苷酸同一性（ANI），该分离株可归属于物种。基因组大小为5.4 Mb，GC含量为59.3%。在基因组中鉴定出了与几丁质酶、铁载体、蛋白酶C、serralisins、溶血素和serrawettin W2合成相关的基因，这些基因已被报道用于线虫生物防治。这是关于具有杀线虫活性的首次报道。基于这些杀线虫活性结果，该菌株可在田间作为辣椒种植中生物防治的替代方法进行评估。
