Laboratoire des Substances Bioactives (LSBA), Centre de Biotechnologie de Borj Cedria, BP-901, 2050, Hammam-Lif, Tunisia,
Dig Dis Sci. 2014 Aug;59(8):1768-78. doi: 10.1007/s10620-014-3128-0. Epub 2014 Apr 6.
Obesity is a tremendous public health problem, characterized by ectopic deposition of fat into non-adipose tissues as liver generating an oxidative stress that could lead to steato-hepatitis. Grape seed and skin extract (GSSE) is a complex mixture of polyphenolics exhibiting robust antioxidative properties.
We hypothesize that GSSE could protect the liver from fat-induced lipotoxicity and have a beneficial effect on liver function.
Hepatoprotective effect of GSSE was measured by using an experimental model of fat-induced rat liver steatosis. Male rats were fed a standard diet or a high-fat diet (HFD) during 6 weeks and treated or not with 500 mg/kg bw GSSE. Lipid deposition into the liver was assessed by triglyceride, cholesterol and phospholipid measurements. Fat-induced lipoperoxidation, carbonylation, depletion of glutathione and of antioxidant enzyme activities were used as oxidative stress markers with a special emphasis on transition metal distribution.
HFD induced liver hypertrophy and inflammation as assessed by high liver transaminases. HFD also induced an oxidative stress characterized by increased lipid and protein oxidation, a drop in glutathione and antioxidant enzyme activities as glutathione peroxidase and superoxide dismutase and a drastic depletion in liver zinc. Importantly, GSSE prevented all the deleterious effects of HFD treatment.
Data suggest that GSSE could be used as a safe preventive agent against fat-induced liver lipotoxicity which could also have potential applications in other non-alcoholic liver diseases.
肥胖是一个严重的公共健康问题，其特征是脂肪异常沉积到非脂肪组织中，如肝脏，产生氧化应激，可能导致脂肪性肝炎。葡萄籽和葡萄皮提取物（GSSE）是一种复杂的多酚混合物，具有强大的抗氧化特性。
我们假设 GSSE 可以保护肝脏免受脂肪引起的脂毒性，并对肝功能有有益的影响。
使用脂肪诱导的大鼠肝脂肪变性实验模型来测量 GSSE 的肝保护作用。雄性大鼠在 6 周内喂食标准饮食或高脂肪饮食（HFD），并用 500mg/kg bw GSSE 进行处理或不处理。通过甘油三酯、胆固醇和磷脂测量来评估肝脏中的脂肪沉积。脂肪诱导的脂质过氧化、羰基化、谷胱甘肽耗竭和抗氧化酶活性的降低被用作氧化应激标志物，特别强调过渡金属分布。
HFD 诱导肝脏肥大和炎症，肝转氨酶升高。HFD 还诱导了氧化应激，表现为脂质和蛋白质氧化增加，谷胱甘肽和抗氧化酶活性（如谷胱甘肽过氧化物酶和超氧化物歧化酶）下降，以及肝脏锌的严重耗竭。重要的是，GSSE 预防了 HFD 治疗的所有不良影响。
数据表明，GSSE 可用作预防脂肪引起的肝毒性的安全剂，也可能在其他非酒精性肝病中有潜在的应用。
