Thirkell D, Myles A D, Russell W C
Department of Biochemistry and Microbiology, University of St. Andrews, Fife, Scotland.
Infect Immun. 1989 Jun;57(6):1697-701. doi: 10.1128/iai.57.6.1697-1701.1989.
The polypeptides of all 14 serotypes of Ureaplasma urealyticum were analyzed by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining. The electrophoretic patterns did not allow ready discrimination of individual serotypes or seroclusters. The analysis of the antigens of serotype 8 was reported previously (B. L. Precious, D. Thirkell, and W. C. Russell, J. Gen. Microbiol. 133:2659-2676, 1987). In this study, three of the surface-expressed membrane antigens of 16, 17, and 96 kilodaltons were further investigated, and monoclonal antibodies were raised against these three polypeptides. The major 96-kilodalton polypeptide was serotype 8 specific, and the 16-kilodalton polypeptide was present only in the larger serocluster. We describe monoclonal antibody probes that unequivocally differentiate serotype 8 from the other serotypes and that separate the two seroclusters of the organism.
采用一维十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和银染法对解脲脲原体的所有14种血清型的多肽进行了分析。电泳图谱无法轻易区分各个血清型或血清簇。血清型8抗原的分析先前已有报道（B. L. 普雷舍斯、D. 瑟克尔和W. C. 拉塞尔，《普通微生物学杂志》133:2659 - 2676, 1987）。在本研究中，对16、17和96千道尔顿的三种表面表达膜抗原进行了进一步研究，并制备了针对这三种多肽的单克隆抗体。主要的96千道尔顿多肽是血清型8特异性的，16千道尔顿多肽仅存在于较大的血清簇中。我们描述了能明确区分血清型8与其他血清型并能区分该生物体两个血清簇的单克隆抗体探针。
