Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, 608002, Tamilnadu, India.
J Physiol Biochem. 2014 Jun;70(2):525-34. doi: 10.1007/s13105-014-0332-5. Epub 2014 Apr 9.
In this study, we have investigated the effect of the nutritive phytochemicals, indole-3-carbinol (I3C) and its metabolite, 3, 3'- diindolylmethane (DIM) on oxidative stress developed in type 2 diabetes mellitus (T2DM). This work was carried out in the genetically modified mouse (C57BL/6J mice) that closely simulated the metabolic abnormalities of the human disease after the administration of high fat diet (HFD). Glucose, insulin, hemoglobin (Hb), glycated hemoglobin (HbA1c), thiobarbituric acid reactive substances (TBARS), lipid hydroperoxides (LOOH), conjugated dienes (CD), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), vitamin C, vitamin E, and reduced glutathione (GSH) levels were monitored in all the groups. Treatments positively modulate the glucose, insulin, and Hb and HbA1c levels in HFD mice. TBARS, LOOH, and CD were decreased in treatment groups when compared to the HFD group. Treatments increase SOD, CAT, GPx levels (erythrocyte, liver, kidney, and heart) and vitamin C, vitamin E, and GSH levels (plasma, liver, kidney, and heart) in diabetic mice. From the study, it was clear that the antioxidant-scavenging action were accelerated in mice treated with DIM than the I3C treatment group which was comparable with the standard drug metformin.
在这项研究中，我们研究了营养植物化学物质吲哚-3-甲醇（I3C）及其代谢物 3,3'-二吲哚甲烷（DIM）对 2 型糖尿病（T2DM）中氧化应激的影响。这项工作是在经过高脂肪饮食（HFD）处理后，在遗传修饰的小鼠（C57BL/6J 小鼠）中进行的，这些小鼠非常模拟人类疾病的代谢异常。在所有组中都监测了葡萄糖、胰岛素、血红蛋白（Hb）、糖化血红蛋白（HbA1c）、硫代巴比妥酸反应物质（TBARS）、脂质过氧化物（LOOH）、共轭二烯（CD）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）、维生素 C、维生素 E 和还原型谷胱甘肽（GSH）的水平。治疗方案可正向调节 HFD 小鼠的葡萄糖、胰岛素和 Hb 及 HbA1c 水平。与 HFD 组相比，治疗组中的 TBARS、LOOH 和 CD 降低。治疗方案可提高糖尿病小鼠红细胞、肝脏、肾脏和心脏中的 SOD、CAT 和 GPx 水平以及血浆、肝脏、肾脏和心脏中的维生素 C、维生素 E 和 GSH 水平。从研究中可以清楚地看出，与 I3C 治疗组相比，DIM 治疗组的抗氧化剂清除作用加快，与标准药物二甲双胍相当。
